Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

F. G. Monroy; J. H. Adams; C. Dobson; I. J. East

doi:10.1016/0014-4894(89)90009-X

Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

F. G. Monroy, J. H. Adams, C. Dobson, I. J. East

Biological Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.

Original language	English (US)
Pages (from-to)	67-73
Number of pages	7
Journal	Experimental Parasitology
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/0014-4894(89)90009-X
State	Published - Jan 1989

Keywords

Adjuvants
Immunity
Nematode
Nematospiroides dubius
Parasite antigens
Vaccines

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/0014-4894(89)90009-X

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title = "Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms",

abstract = "Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.",

keywords = "Adjuvants, Immunity, Nematode, Nematospiroides dubius, Parasite antigens, Vaccines",

author = "Monroy, {F. G.} and Adams, {J. H.} and C. Dobson and East, {I. J.}",

note = "Funding Information: This work was supported by a CSIRO/University of Queensland collaborative research fund and the Australian research grant scheme.",

year = "1989",

month = jan,

doi = "10.1016/0014-4894(89)90009-X",

language = "English (US)",

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AU - Monroy, F. G.

AU - Adams, J. H.

AU - Dobson, C.

AU - East, I. J.

N1 - Funding Information: This work was supported by a CSIRO/University of Queensland collaborative research fund and the Australian research grant scheme.

PY - 1989/1

Y1 - 1989/1

N2 - Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.

AB - Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.

KW - Adjuvants

KW - Immunity

KW - Nematode

KW - Nematospiroides dubius

KW - Parasite antigens

KW - Vaccines

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Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

Abstract

Keywords

ASJC Scopus subject areas

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