TY - GEN
T1 - NanoCluster beacon - A new molecular probe for homogeneous detection of nucleic acid targets
AU - Yeh, Hsin Chih
AU - Sharma, Jaswinder
AU - Han, Jason J.
AU - Martinez, Jennifer S.
AU - Werner, James H.
PY - 2011
Y1 - 2011
N2 - Oligonucleotide-templated nanoclusters consisting of a few atoms of silver (DNA/Ag NCs) have been made into a new molecular probe that "lights up" upon target DNA binding, termed a NanoCluster Beacon (NCB). We discovered that interactions between silver nanoclusters and a proximal, guanine-rich DNA strand can lead to tremendous red fluorescence enhancement [1]. Here we show that dark silver nanoclusters templated on an ssDNA can be lit up into a palette of colors (green, yellow/orange, and red) by employing different proximal sequences, potentially enabling the use of NCBs in multiplexed assays. We tested a suite of nanocluster-nucleation sequences and found the sequences that created strong red fluorescence enhancement share a common 5'-C 3NNNNNC4 motif, where N is either a thymine (T) or an adenine (A) base. We optimized the design of NCBs by testing the effect of different lengths of an interaction stem. The highest signal-to-background (S/B) ratio, 175, was achieved when the stem length was 3 base pairs long.
AB - Oligonucleotide-templated nanoclusters consisting of a few atoms of silver (DNA/Ag NCs) have been made into a new molecular probe that "lights up" upon target DNA binding, termed a NanoCluster Beacon (NCB). We discovered that interactions between silver nanoclusters and a proximal, guanine-rich DNA strand can lead to tremendous red fluorescence enhancement [1]. Here we show that dark silver nanoclusters templated on an ssDNA can be lit up into a palette of colors (green, yellow/orange, and red) by employing different proximal sequences, potentially enabling the use of NCBs in multiplexed assays. We tested a suite of nanocluster-nucleation sequences and found the sequences that created strong red fluorescence enhancement share a common 5'-C 3NNNNNC4 motif, where N is either a thymine (T) or an adenine (A) base. We optimized the design of NCBs by testing the effect of different lengths of an interaction stem. The highest signal-to-background (S/B) ratio, 175, was achieved when the stem length was 3 base pairs long.
KW - DNA-templated silver nanoclusters
KW - Noble metal nanoclusters
KW - fluorescent probes
KW - light-up probes
UR - http://www.scopus.com/inward/record.url?scp=80053340003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053340003&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017345
DO - 10.1109/NEMS.2011.6017345
M3 - Conference contribution
AN - SCOPUS:80053340003
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 267
EP - 270
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -