TY - JOUR
T1 - Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA
AU - Engelthaler, David M.
AU - Bowers, Jolene
AU - Schupp, James A.
AU - Pearson, Talima
AU - Ginther, Jennifer
AU - Hornstra, Heidie M.
AU - Dale, Julia
AU - Stewart, Tasha
AU - Sunenshine, Rebecca
AU - Waddell, Victor
AU - Levy, Craig
AU - Gillece, John
AU - Price, Lance B.
AU - Contente, Tania
AU - Beckstrom-Sternberg, Stephen M.
AU - Blaney, David D.
AU - Wagner, David M.
AU - Mayo, Mark
AU - Currie, Bart J.
AU - Keim, Paul
AU - Tuanyok, Apichai
PY - 2011/10
Y1 - 2011/10
N2 - Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification.
AB - Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification.
UR - http://www.scopus.com/inward/record.url?scp=80055087611&partnerID=8YFLogxK
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U2 - 10.1371/journal.pntd.0001347
DO - 10.1371/journal.pntd.0001347
M3 - Article
C2 - 22028940
AN - SCOPUS:80055087611
SN - 1935-2727
VL - 5
JO - PLoS neglected tropical diseases
JF - PLoS neglected tropical diseases
IS - 10
M1 - e1347
ER -