TY - JOUR
T1 - Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus
AU - Vogler, Amy J.
AU - Busch, Joseph D.
AU - Percy-Fine, Stephanie
AU - Tipton-Hunton, Christine
AU - Smith, Kimothy L.
AU - Keim, Paul
PY - 2002
Y1 - 2002
N2 - Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 × 10-9 mutations/generation by a Luria-Delbrück fluctuation test.
AB - Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 × 10-9 mutations/generation by a Luria-Delbrück fluctuation test.
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U2 - 10.1128/AAC.46.2.511-513.2002
DO - 10.1128/AAC.46.2.511-513.2002
M3 - Article
C2 - 11796364
AN - SCOPUS:0036148306
SN - 0066-4804
VL - 46
SP - 511
EP - 513
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 2
ER -