Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii

Ayush Kumar, Courtney Dalton, Jenny Cortez-Cordova, Herbert P. Schweizer

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn. 7-based single copy insertion system in A. baumannii. Mini-Tn. 7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosamine-fructose-6-phosphate aminotransferase) in Gram-negative bacteria. We identified the site of insertion of mini-Tn. 7 in A. baumannii and demonstrated application of this useful cloning tool by inserting the gfp gene into the chromosome. Our work shows that mini-Tn 7 elements are useful tools for genetic studies in this important pathogen.

Original languageEnglish (US)
Pages (from-to)296-300
Number of pages5
JournalJournal of Microbiological Methods
Volume82
Issue number3
DOIs
StatePublished - Sep 2010
Externally publishedYes

Keywords

  • A. baumannii
  • Mini-Tn7
  • Single-copy gene cloning

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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