Micropatterned avidin arrays on silicon substrates via photolithography, self-assembly and bioconjugation

Timothy L. Vail, Kevin W. Cushing, Jani C. Ingram, Ingrid St. Omer

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self-assembly. Selective capping of carboxy groups on alkanethiols by N-hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In-process characterization methods included grazing-angle Fourier-transform IR spectroscopy, ellipsometry, contact-angle goniometry, atomic-force microscopy and fluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalBiotechnology and Applied Biochemistry
Issue number2
StatePublished - Feb 2006


  • Alkanethiol
  • Avidin
  • Biotin
  • Microarray
  • Quantum dots
  • Self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology


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