Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b

Ayush Kumar; Kim Lee Chua; Herbert P. Schweizer

doi:10.1128/AAC.00440-06

Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b

Ayush Kumar
, Kim Lee Chua
, Herbert P. Schweizer

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.

Original language	English (US)
Pages (from-to)	3460-3463
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	50
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00440-06
State	Published - Oct 2006
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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Kumar, A., Chua, K. L., & Schweizer, H. P. (2006). Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrobial Agents and Chemotherapy, 50(10), 3460-3463. https://doi.org/10.1128/AAC.00440-06

Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. / Kumar, Ayush; Chua, Kim Lee; Schweizer, Herbert P.
In: Antimicrobial Agents and Chemotherapy, Vol. 50, No. 10, 10.2006, p. 3460-3463.

Research output: Contribution to journal › Article › peer-review

Kumar, A, Chua, KL & Schweizer, HP 2006, 'Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b', Antimicrobial Agents and Chemotherapy, vol. 50, no. 10, pp. 3460-3463. https://doi.org/10.1128/AAC.00440-06

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abstract = "Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.",

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Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b

Abstract

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