TY - JOUR
T1 - Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain
T2 - Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b
AU - Kumar, Ayush
AU - Chua, Kim Lee
AU - Schweizer, Herbert P.
PY - 2006/10
Y1 - 2006/10
N2 - Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.
AB - Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.
UR - http://www.scopus.com/inward/record.url?scp=33749511442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749511442&partnerID=8YFLogxK
U2 - 10.1128/AAC.00440-06
DO - 10.1128/AAC.00440-06
M3 - Article
C2 - 17005832
AN - SCOPUS:33749511442
SN - 0066-4804
VL - 50
SP - 3460
EP - 3463
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 10
ER -