Mammalian stem cells reprogramming in response to terahertz radiation

Jonathan Bock, Yayoi Fukuyo, Sona Kang, M. Lisa Phipps, Ludmil B. Alexandrov, Kim O. Rasmussen, Alan R. Bishop, Evan D. Rosen, Jennifer S. Martinez, Hou Tong Chen, George Rodriguez, Boian S. Alexandrov, Anny Usheva

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

We report that extended exposure to broad-spectrum terahertz radiation results in specific changes in cellular functions that are closely related to DNA-directed gene transcription. Our gene chip survey of gene expression shows that whereas 89% of the protein coding genes in mouse stem cells do not respond to the applied terahertz radiation, certain genes are activated, while other are repressed. RT-PCR experiments with selected gene probes corresponding to transcripts in the three groups of genes detail the gene specific effect. The response was not only gene specific but also irradiation conditions dependent. Our findings suggest that the applied terahertz irradiation accelerates cell differentiation toward adipose phenotype by activating the transcription factor peroxisome proliferator-activated receptor gamma (PPARG). Finally, our molecular dynamics computer simulations indicate that the local breathing dynamics of the PPARG promoter DNA coincides with the gene specific response to the THz radiation. We propose that THz radiation is a potential tool for cellular reprogramming.

Original languageEnglish (US)
Article numbere15806
JournalPLoS ONE
Volume5
Issue number12
DOIs
StatePublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • General

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