LupiQuant: A real-time PCR based assay for determining host-to-parasite DNA ratios of Onchocerca lupi and host Canis lupus from onchocercosis samples

Keyphrases

• Quantitative PCR 100%
• Whole Genome Sequencing 100%
• DNA Concentration 100%
• QPCR Assay 100%
• Canis Lupus 100%
• Onchocercosis 100%
• Onchocerca Lupi 100%
• Parasite DNA 100%
• Canine 80%
• Host DNA 80%
• Myosin 60%
• Plasmid 40%
• Single-copy Gene 40%
• Gene-specific 40%
• Onchocerca 40%
• PKD1 40%
• North America 20%
• Middle East 20%
• Europe 20%
• Whole Genome 20%
• Copy number 20%
• One Sample 20%
• Whole-genome Sequence Data 20%
• Gene Content 20%
• Quantification Assay 20%
• Early 21st Century 20%
• DNA Extraction 20%
• Draft Sequence 20%
• Range Expansion 20%
• Accurate Method 20%
• Sequencing Studies 20%
• Genome Draft 20%
• Genome Copy number 20%
• North Africa 20%
• Overabundance 20%
• Variable Quantity 20%
• Filarial Nematodes 20%
• Future-focused 20%
• Sample Selection 20%
• North Area 20%
• Body Wall 20%
• Polycystin 20%
• DNA Load 20%
• Reported Incidence 20%

Immunology and Microbiology

• Real Time Polymerase Chain Reaction 100%
• Whole Genome Sequencing 100%
• Onchocerca 100%
• Onchocerciasis 100%
• Canis Lupus 100%
• Plasmid 40%
• Real-Time Polymerase Chain Reaction 20%
• Nematode 20%
• DNA Extraction 20%
• Precursor 20%
• Sampling 20%