Long RT-PCR of the entire 8.5-kb NF1 open reading frame and mutation detection on agarose gels

Keyphrases

• Upstream Open Reading Frame (uORF) 100%
• Long RT-PCR 100%
• Frame Detection 100%
• Agarose Gel 100%
• Mutation Detection 100%
• Neurofibromatosis Type 1 (NF-1) 100%
• Exon 80%
• PCR Products 40%
• Quantitative PCR 20%
• Deletion mutation 20%
• Dropout 20%
• PCR Primers 20%
• Binding Site 20%
• Protein-coding Genes 20%
• Insertion-deletion 20%
• PCR Amplification 20%
• Complementary DNA (cDNA) 20%
• Agarose Gel Electrophoresis 20%
• Cause of Disease 20%
• Restriction Digestion 20%
• Whole Blood 20%
• Riots 20%
• Gel Analysis 20%
• Primer Binding Site 20%
• Lymphoblast 20%
• Total RNA 20%
• Simple Assay 20%
• Patient mutations 20%
• Single-cell PCR 20%

Biochemistry, Genetics and Molecular Biology

• Reverse Transcription Polymerase Chain Reaction 100%
• Open Reading Frame 100%
• Exon 100%
• Agarose 100%
• Polymerase Chain Reaction 75%
• Allele 50%
• Binding Site 50%
• Messenger RNA 25%
• Indel 25%
• Agarose Gel Electrophoresis 25%
• RNA 25%
• Restriction Modification System 25%
• Lymphoblast 25%