Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico

Luis G.V. Fernandes; Nathan E. Stone; Chandler C. Roe; Marga G.A. Goris; Hans van der Linden; Jason W. Sahl; David M. Wagner; Jarlath E. Nally

doi:10.1099/IJSEM.0.005560

Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico

Luis G.V. Fernandes
, Nathan E. Stone
, Chandler C. Roe
, Marga G.A. Goris
, Hans van der Linden
, Jason W. Sahl
, David M. Wagner
, Jarlath E. Nally

Biological Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Two spirochetes (designated strains LGVF01 and LGVF02^T) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2%, but below 93.2% when compared to any previously described leptospiral species. Serotyping of strain LGVF02^T indicates that it does not belong within any sero-group of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02^T represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02^T (=NVSL-LGVF02^T=KIT0302^T).

Original language	English (US)
Article number	005560
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	72
Issue number	10
DOIs	https://doi.org/10.1099/IJSEM.0.005560
State	Published - Oct 19 2022

Keywords

Leptospira
leptospirosis
pathogenic
soil
whole-genome sequencing

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/IJSEM.0.005560

Cite this

Fernandes, L. G. V., Stone, N. E., Roe, C. C., Goris, M. G. A., van der Linden, H., Sahl, J. W., Wagner, D. M., & Nally, J. E. (2022). Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico. International Journal of Systematic and Evolutionary Microbiology, 72(10), Article 005560. https://doi.org/10.1099/IJSEM.0.005560

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title = "Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico",

abstract = "Two spirochetes (designated strains LGVF01 and LGVF02T) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2\%, but below 93.2\% when compared to any previously described leptospiral species. Serotyping of strain LGVF02T indicates that it does not belong within any sero-group of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02T represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02T (=NVSL-LGVF02T=KIT0302T).",

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doi = "10.1099/IJSEM.0.005560",

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AU - Fernandes, Luis G.V.

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AU - Roe, Chandler C.

AU - Goris, Marga G.A.

AU - van der Linden, Hans

AU - Sahl, Jason W.

AU - Wagner, David M.

AU - Nally, Jarlath E.

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N2 - Two spirochetes (designated strains LGVF01 and LGVF02T) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2%, but below 93.2% when compared to any previously described leptospiral species. Serotyping of strain LGVF02T indicates that it does not belong within any sero-group of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02T represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02T (=NVSL-LGVF02T=KIT0302T).

AB - Two spirochetes (designated strains LGVF01 and LGVF02T) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2%, but below 93.2% when compared to any previously described leptospiral species. Serotyping of strain LGVF02T indicates that it does not belong within any sero-group of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02T represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02T (=NVSL-LGVF02T=KIT0302T).

KW - Leptospira

KW - leptospirosis

KW - pathogenic

KW - soil

KW - whole-genome sequencing

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Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico

Abstract

Keywords

ASJC Scopus subject areas

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