Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Patrick D. Schloss; Sarah L. Westcott; Thomas Ryabin; Justine R. Hall; Martin Hartmann; Emily B. Hollister; Ryan A. Lesniewski; Brian B. Oakley; Donovan H. Parks; Courtney J. Robinson; Jason W. Sahl; Blaz Stres; Gerhard G. Thallinger; David J. Van Horn; Carolyn F. Weber

doi:10.1128/AEM.01541-09

Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Patrick D. Schloss
, Sarah L. Westcott
, Thomas Ryabin
, Justine R. Hall
, Martin Hartmann
, Emily B. Hollister
, Ryan A. Lesniewski
, Brian B. Oakley
, Donovan H. Parks
, Courtney J. Robinson
, Jason W. Sahl
, Blaz Stres
, Gerhard G. Thallinger
, David J. Van Horn
, Carolyn F. Weber

Research output: Contribution to journal › Article › peer-review

18083 Scopus citations

Abstract

Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

Original language	English (US)
Pages (from-to)	7537-7541
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	23
DOIs	https://doi.org/10.1128/AEM.01541-09
State	Published - Dec 2009
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., Lesniewski, R. A., Oakley, B. B., Parks, D. H., Robinson, C. J., Sahl, J. W., Stres, B., Thallinger, G. G., Van Horn, D. J., & Weber, C. F. (2009). Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75(23), 7537-7541. https://doi.org/10.1128/AEM.01541-09

Schloss, PD, Westcott, SL, Ryabin, T, Hall, JR, Hartmann, M, Hollister, EB, Lesniewski, RA, Oakley, BB, Parks, DH, Robinson, CJ, Sahl, JW, Stres, B, Thallinger, GG, Van Horn, DJ & Weber, CF 2009, 'Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities', Applied and Environmental Microbiology, vol. 75, no. 23, pp. 7537-7541. https://doi.org/10.1128/AEM.01541-09

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abstract = "Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.",

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AU - Parks, Donovan H.

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AU - Sahl, Jason W.

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Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Abstract

ASJC Scopus subject areas

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