Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Patrick D. Schloss; Sarah L. Westcott; Thomas Ryabin; Justine R. Hall; Martin Hartmann; Emily B. Hollister; Ryan A. Lesniewski; Brian B. Oakley; Donovan H. Parks; Courtney J. Robinson; Jason W. Sahl; Blaz Stres; Gerhard G. Thallinger; David J. Van Horn; Carolyn F. Weber

doi:10.1128/AEM.01541-09

Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Patrick D. Schloss, Sarah L. Westcott, Thomas Ryabin, Justine R. Hall, Martin Hartmann, Emily B. Hollister, Ryan A. Lesniewski, Brian B. Oakley, Donovan H. Parks, Courtney J. Robinson, Jason W. Sahl, Blaz Stres, Gerhard G. Thallinger, David J. Van Horn, Carolyn F. Weber

Research output: Contribution to journal › Article › peer-review

17782 Scopus citations

Abstract

Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

Original language	English (US)
Pages (from-to)	7537-7541
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	23
DOIs	https://doi.org/10.1128/AEM.01541-09
State	Published - Dec 2009
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

Access to Document

10.1128/AEM.01541-09

Cite this

Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., Lesniewski, R. A., Oakley, B. B., Parks, D. H., Robinson, C. J., Sahl, J. W., Stres, B., Thallinger, G. G., Van Horn, D. J., & Weber, C. F. (2009). Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75(23), 7537-7541. https://doi.org/10.1128/AEM.01541-09

Schloss, PD, Westcott, SL, Ryabin, T, Hall, JR, Hartmann, M, Hollister, EB, Lesniewski, RA, Oakley, BB, Parks, DH, Robinson, CJ, Sahl, JW, Stres, B, Thallinger, GG, Van Horn, DJ & Weber, CF 2009, 'Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities', Applied and Environmental Microbiology, vol. 75, no. 23, pp. 7537-7541. https://doi.org/10.1128/AEM.01541-09

@article{a9126d6274424add910961c151d1f150,

title = "Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities",

abstract = "Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.",

author = "Schloss, \{Patrick D.\} and Westcott, \{Sarah L.\} and Thomas Ryabin and Hall, \{Justine R.\} and Martin Hartmann and Hollister, \{Emily B.\} and Lesniewski, \{Ryan A.\} and Oakley, \{Brian B.\} and Parks, \{Donovan H.\} and Robinson, \{Courtney J.\} and Sahl, \{Jason W.\} and Blaz Stres and Thallinger, \{Gerhard G.\} and \{Van Horn\}, \{David J.\} and Weber, \{Carolyn F.\}",

year = "2009",

month = dec,

doi = "10.1128/AEM.01541-09",

language = "English (US)",

volume = "75",

pages = "7537--7541",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "23",

}

TY - JOUR

T1 - Introducing mothur

T2 - Open-source, platform-independent, community-supported software for describing and comparing microbial communities

AU - Schloss, Patrick D.

AU - Westcott, Sarah L.

AU - Ryabin, Thomas

AU - Hall, Justine R.

AU - Hartmann, Martin

AU - Hollister, Emily B.

AU - Lesniewski, Ryan A.

AU - Oakley, Brian B.

AU - Parks, Donovan H.

AU - Robinson, Courtney J.

AU - Sahl, Jason W.

AU - Stres, Blaz

AU - Thallinger, Gerhard G.

AU - Van Horn, David J.

AU - Weber, Carolyn F.

PY - 2009/12

Y1 - 2009/12

N2 - Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

AB - Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

UR - https://www.scopus.com/pages/publications/72949107142

UR - https://www.scopus.com/inward/citedby.url?scp=72949107142&partnerID=8YFLogxK

U2 - 10.1128/AEM.01541-09

DO - 10.1128/AEM.01541-09

M3 - Article

C2 - 19801464

AN - SCOPUS:72949107142

SN - 0099-2240

VL - 75

SP - 7537

EP - 7541

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 23

ER -

Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this