TY - JOUR
T1 - Identification and genetic characterization of the Pseudomonas aeruginosa leuB gene encoding 3-isopropylmalate dehydrogenase
AU - Hoang, T. T.
AU - Schweizer, H. P.
N1 - Funding Information:
Acknowledgments We wish to thank B.H. Holloway and P.V. Phibbs for the gifts of strains and cosmids. The initial stages of this research were supported by a grant from the Canadian Bacterial Diseases Network, by a Medical Scholorship from the Medical Research Council of Canada to H.P.S., and subsequently by funds from Colorado State University.
PY - 1997
Y1 - 1997
N2 - The Pseudomonas aeruginosa leuB gene, encoding 3-isopropylmalate dehydrogenase, was identified upstream of asd, encoding aspartate-β-semialdehyde dehydrogenase. Genetic analysis indicated that leuB is identical to the previously mapped gene defined by the leu-10 allele. The chromosomal leuB locus was inactivated by gene replacement. The insertions had no adverse effect on expression of the downstream asd gene but resulted in leucine auxotrophy. The leuB gene encodes a protein containing 360 amino acids (with a molecular weight of 39153), which was expressed in Escherichia coli as a M, 42000 protein. The results suggested that, in contrast to the situation in other bacteria (E. coli, Salmonella typhimurium and Bacillus subtilis) the P. aeruginosa leuB gene is physically separated from the genes encoding the other enzymes of the isopropylmalate pathway.
AB - The Pseudomonas aeruginosa leuB gene, encoding 3-isopropylmalate dehydrogenase, was identified upstream of asd, encoding aspartate-β-semialdehyde dehydrogenase. Genetic analysis indicated that leuB is identical to the previously mapped gene defined by the leu-10 allele. The chromosomal leuB locus was inactivated by gene replacement. The insertions had no adverse effect on expression of the downstream asd gene but resulted in leucine auxotrophy. The leuB gene encodes a protein containing 360 amino acids (with a molecular weight of 39153), which was expressed in Escherichia coli as a M, 42000 protein. The results suggested that, in contrast to the situation in other bacteria (E. coli, Salmonella typhimurium and Bacillus subtilis) the P. aeruginosa leuB gene is physically separated from the genes encoding the other enzymes of the isopropylmalate pathway.
KW - 3-isopropylmalate dehydrogenase
KW - Cloning
KW - Genetic analysis
KW - Leucine biosynthesis
KW - Pseudomonas aeruginosa
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U2 - 10.1007/s004380050404
DO - 10.1007/s004380050404
M3 - Article
C2 - 9108278
AN - SCOPUS:0030932588
SN - 0026-8925
VL - 254
SP - 166
EP - 170
JO - Molecular and General Genetics
JF - Molecular and General Genetics
IS - 2
ER -