Identification and genetic characterization of the Pseudomonas aeruginosa leuB gene encoding 3-isopropylmalate dehydrogenase

T. T. Hoang, H. P. Schweizer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The Pseudomonas aeruginosa leuB gene, encoding 3-isopropylmalate dehydrogenase, was identified upstream of asd, encoding aspartate-β-semialdehyde dehydrogenase. Genetic analysis indicated that leuB is identical to the previously mapped gene defined by the leu-10 allele. The chromosomal leuB locus was inactivated by gene replacement. The insertions had no adverse effect on expression of the downstream asd gene but resulted in leucine auxotrophy. The leuB gene encodes a protein containing 360 amino acids (with a molecular weight of 39153), which was expressed in Escherichia coli as a M, 42000 protein. The results suggested that, in contrast to the situation in other bacteria (E. coli, Salmonella typhimurium and Bacillus subtilis) the P. aeruginosa leuB gene is physically separated from the genes encoding the other enzymes of the isopropylmalate pathway.

Original languageEnglish (US)
Pages (from-to)166-170
Number of pages5
JournalMolecular and General Genetics
Volume254
Issue number2
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • 3-isopropylmalate dehydrogenase
  • Cloning
  • Genetic analysis
  • Leucine biosynthesis
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Genetics

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