Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

Herbert P. Schweizer; Prasanta Datta

doi:10.1007/BF00332418

Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

Herbert P. Schweizer, Prasanta Datta

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20 Scopus citations

Abstract

Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that is is a polypeptide of M_r 12000 with 99 amino acid residues and contains a potential helix-turnhelix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.

Original language	English (US)
Pages (from-to)	516-522
Number of pages	7
Journal	MGG Molecular & General Genetics
Volume	218
Issue number	3
DOIs	https://doi.org/10.1007/BF00332418
State	Published - Sep 1989
Externally published	Yes

Keywords

Escherichia coli
Gene regulation
tdc activator
tdc operon

ASJC Scopus subject areas

Genetics

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10.1007/BF00332418

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title = "Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli",

abstract = "Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that is is a polypeptide of Mr 12000 with 99 amino acid residues and contains a potential helix-turnhelix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.",

keywords = "Escherichia coli, Gene regulation, tdc activator, tdc operon",

author = "Schweizer, {Herbert P.} and Prasanta Datta",

year = "1989",

month = sep,

doi = "10.1007/BF00332418",

language = "English (US)",

volume = "218",

pages = "516--522",

journal = "MGG Molecular & General Genetics",

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publisher = "Springer Science and Business Media Deutschland GmbH",

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TY - JOUR

T1 - Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

AU - Schweizer, Herbert P.

AU - Datta, Prasanta

PY - 1989/9

Y1 - 1989/9

N2 - Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that is is a polypeptide of Mr 12000 with 99 amino acid residues and contains a potential helix-turnhelix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.

AB - Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that is is a polypeptide of Mr 12000 with 99 amino acid residues and contains a potential helix-turnhelix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.

KW - Escherichia coli

KW - Gene regulation

KW - tdc activator

KW - tdc operon

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DO - 10.1007/BF00332418

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AN - SCOPUS:0024441334

SN - 0026-8925

VL - 218

SP - 516

EP - 522

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 3

ER -

Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

Abstract

Keywords

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