Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli

Herbert P. Schweizer, Prasanta Datta

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that is is a polypeptide of Mr 12000 with 99 amino acid residues and contains a potential helix-turnhelix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.

Original languageEnglish (US)
Pages (from-to)516-522
Number of pages7
JournalMGG Molecular & General Genetics
Volume218
Issue number3
DOIs
StatePublished - Sep 1989
Externally publishedYes

Keywords

  • Escherichia coli
  • Gene regulation
  • tdc activator
  • tdc operon

ASJC Scopus subject areas

  • Genetics

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