TY - JOUR
T1 - High-resolution analytical imaging and electron holography of magnetite particles in amyloid cores of Alzheimerâ €™ s disease
AU - Plascencia-Villa, Germán
AU - Ponce, Arturo
AU - Collingwood, Joanna F.
AU - Josefina Arellano-Jiménez, M.
AU - Zhu, Xiongwei
AU - Rogers, Jack T.
AU - Betancourt, Israel
AU - José-Yacamán, Miguel
AU - Perry, George
N1 - Funding Information:
This work was supported by Welch Foundation (AX-1615), NSF (DMR-1103730), NIH RCMI (5G12RR013646-12 and G12MD007591) and Semmes Foundation. Technical assistance of Sandra Siedlak. Facilities of Department of Pathology, Case Western Reserve University. Facilities of Kleberg Advanced Microscopy Center (KAMiC), NIH RCMI Nanotechnology and Human Health Core and NIH RCMI Biophotonics Core at UTSA. We dedicate this paper to Mark A. Smith for first noting the magnetic properties of APC.
PY - 2016/4/28
Y1 - 2016/4/28
N2 - Abnormal accumulation of brain metals is a key feature of Alzheimer's disease (AD). Formation of amyloid-β plaque cores (APC) is related to interactions with biometals, especially Fe, Cu and Zn, but their particular structural associations and roles remain unclear. Using an integrative set of advanced transmission electron microscopy (TEM) techniques, including spherical aberration-corrected scanning transmission electron microscopy (Cs-STEM), nano-beam electron diffraction, electron holography and analytical spectroscopy techniques (EDX and EELS), we demonstrate that Fe in APC is present as iron oxide (Fe3 O4) magnetite nanoparticles. Here we show that Fe was accumulated primarily as nanostructured particles within APC, whereas Cu and Zn were distributed through the amyloid fibers. Remarkably, these highly organized crystalline magnetite nanostructures directly bound into fibrillar Aβ showed characteristic superparamagnetic responses with saturated magnetization with circular contours, as observed for the first time by off-axis electron holography of nanometer scale particles.
AB - Abnormal accumulation of brain metals is a key feature of Alzheimer's disease (AD). Formation of amyloid-β plaque cores (APC) is related to interactions with biometals, especially Fe, Cu and Zn, but their particular structural associations and roles remain unclear. Using an integrative set of advanced transmission electron microscopy (TEM) techniques, including spherical aberration-corrected scanning transmission electron microscopy (Cs-STEM), nano-beam electron diffraction, electron holography and analytical spectroscopy techniques (EDX and EELS), we demonstrate that Fe in APC is present as iron oxide (Fe3 O4) magnetite nanoparticles. Here we show that Fe was accumulated primarily as nanostructured particles within APC, whereas Cu and Zn were distributed through the amyloid fibers. Remarkably, these highly organized crystalline magnetite nanostructures directly bound into fibrillar Aβ showed characteristic superparamagnetic responses with saturated magnetization with circular contours, as observed for the first time by off-axis electron holography of nanometer scale particles.
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U2 - 10.1038/srep24873
DO - 10.1038/srep24873
M3 - Article
C2 - 27121137
AN - SCOPUS:84964916171
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 24873
ER -