Genomic diversity of the human pathogen Paracoccidioides across the South American continent

Marcus de Melo Teixeira; Maria Emilia Cattana; Daniel R. Matute; José F. Muñoz; Alicia Arechavala; Kristin Isbell; Rafael Schipper; Gabriela Santiso; Fernanda Tracogna; María de los Ángeles Sosa; Norma Cech; Primavera Alvarado; Laura Barreto; Yone Chacón; Juana Ortellado; Cleoni Mendes de Lima; Marilene Rodrigues Chang; Gustavo Niño-Vega; Maria Aparecida Shikanai Yasuda; Maria Sueli Soares Felipe; Ricardo Negroni; Christina A. Cuomo; Bridget Barker; Gustavo Giusiano

doi:10.1016/j.fgb.2020.103395

Genomic diversity of the human pathogen Paracoccidioides across the South American continent

Marcus de Melo Teixeira, Maria Emilia Cattana, Daniel R. Matute, José F. Muñoz, Alicia Arechavala, Kristin Isbell, Rafael Schipper, Gabriela Santiso, Fernanda Tracogna, María de los Ángeles Sosa, Norma Cech, Primavera Alvarado, Laura Barreto, Yone Chacón, Juana Ortellado, Cleoni Mendes de Lima, Marilene Rodrigues Chang, Gustavo Niño-Vega, Maria Aparecida Shikanai Yasuda, Maria Sueli Soares FelipeRicardo Negroni, Christina A. Cuomo, Bridget Barker, Gustavo Giusiano

Biological Sciences

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Paracoccidioidomycosis (PCM) is a life-threatening systemic mycosis widely reported in the Gran Chaco ecosystem. The disease is caused by different species from the genus Paracoccidioides, which are all endemic to South and Central America. Here, we sequenced and analyzed 31 isolates of Paracoccidioides across South America, with particular focus on isolates from Argentina and Paraguay. The de novo sequenced isolates were compared with publicly available genomes. Phylogenetics and population genomics revealed that PCM in Argentina and Paraguay is caused by three distinct Paracoccidioides genotypes, P. brasiliensis (S1a and S1b) and P. restrepiensis (PS3). P. brasiliensis S1a isolates from Argentina are frequently associated with chronic forms of the disease. Our results suggest the existence of extensive molecular polymorphism among Paracoccidioides species, and provide a framework to begin to dissect the connection between genotypic differences in the pathogen and the clinical outcomes of the disease.

Original language	English (US)
Article number	103395
Journal	Fungal Genetics and Biology
Volume	140
DOIs	https://doi.org/10.1016/j.fgb.2020.103395
State	Published - Jul 2020

Keywords

Gran Chaco
Paracoccidioides
Phylogenomics
Population genetics

ASJC Scopus subject areas

Microbiology
Genetics

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10.1016/j.fgb.2020.103395

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Teixeira, M. D. M., Cattana, M. E., Matute, D. R., Muñoz, J. F., Arechavala, A., Isbell, K., Schipper, R., Santiso, G., Tracogna, F., Sosa, M. D. L. Á., Cech, N., Alvarado, P., Barreto, L., Chacón, Y., Ortellado, J., Lima, C. M. D., Chang, M. R., Niño-Vega, G., Yasuda, M. A. S., ... Giusiano, G. (2020). Genomic diversity of the human pathogen Paracoccidioides across the South American continent. Fungal Genetics and Biology, 140, Article 103395. https://doi.org/10.1016/j.fgb.2020.103395

Teixeira, MDM, Cattana, ME, Matute, DR, Muñoz, JF, Arechavala, A, Isbell, K, Schipper, R, Santiso, G, Tracogna, F, Sosa, MDLÁ, Cech, N, Alvarado, P, Barreto, L, Chacón, Y, Ortellado, J, Lima, CMD, Chang, MR, Niño-Vega, G, Yasuda, MAS, Felipe, MSS, Negroni, R, Cuomo, CA, Barker, B & Giusiano, G 2020, 'Genomic diversity of the human pathogen Paracoccidioides across the South American continent', Fungal Genetics and Biology, vol. 140, 103395. https://doi.org/10.1016/j.fgb.2020.103395

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title = "Genomic diversity of the human pathogen Paracoccidioides across the South American continent",

abstract = "Paracoccidioidomycosis (PCM) is a life-threatening systemic mycosis widely reported in the Gran Chaco ecosystem. The disease is caused by different species from the genus Paracoccidioides, which are all endemic to South and Central America. Here, we sequenced and analyzed 31 isolates of Paracoccidioides across South America, with particular focus on isolates from Argentina and Paraguay. The de novo sequenced isolates were compared with publicly available genomes. Phylogenetics and population genomics revealed that PCM in Argentina and Paraguay is caused by three distinct Paracoccidioides genotypes, P. brasiliensis (S1a and S1b) and P. restrepiensis (PS3). P. brasiliensis S1a isolates from Argentina are frequently associated with chronic forms of the disease. Our results suggest the existence of extensive molecular polymorphism among Paracoccidioides species, and provide a framework to begin to dissect the connection between genotypic differences in the pathogen and the clinical outcomes of the disease.",

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year = "2020",

month = jul,

doi = "10.1016/j.fgb.2020.103395",

language = "English (US)",

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AU - Teixeira, Marcus de Melo

AU - Cattana, Maria Emilia

AU - Matute, Daniel R.

AU - Muñoz, José F.

AU - Arechavala, Alicia

AU - Isbell, Kristin

AU - Schipper, Rafael

AU - Santiso, Gabriela

AU - Tracogna, Fernanda

AU - Sosa, María de los Ángeles

AU - Cech, Norma

AU - Alvarado, Primavera

AU - Barreto, Laura

AU - Chacón, Yone

AU - Ortellado, Juana

AU - Lima, Cleoni Mendes de

AU - Chang, Marilene Rodrigues

AU - Niño-Vega, Gustavo

AU - Yasuda, Maria Aparecida Shikanai

AU - Felipe, Maria Sueli Soares

AU - Negroni, Ricardo

AU - Cuomo, Christina A.

AU - Barker, Bridget

AU - Giusiano, Gustavo

PY - 2020/7

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N2 - Paracoccidioidomycosis (PCM) is a life-threatening systemic mycosis widely reported in the Gran Chaco ecosystem. The disease is caused by different species from the genus Paracoccidioides, which are all endemic to South and Central America. Here, we sequenced and analyzed 31 isolates of Paracoccidioides across South America, with particular focus on isolates from Argentina and Paraguay. The de novo sequenced isolates were compared with publicly available genomes. Phylogenetics and population genomics revealed that PCM in Argentina and Paraguay is caused by three distinct Paracoccidioides genotypes, P. brasiliensis (S1a and S1b) and P. restrepiensis (PS3). P. brasiliensis S1a isolates from Argentina are frequently associated with chronic forms of the disease. Our results suggest the existence of extensive molecular polymorphism among Paracoccidioides species, and provide a framework to begin to dissect the connection between genotypic differences in the pathogen and the clinical outcomes of the disease.

AB - Paracoccidioidomycosis (PCM) is a life-threatening systemic mycosis widely reported in the Gran Chaco ecosystem. The disease is caused by different species from the genus Paracoccidioides, which are all endemic to South and Central America. Here, we sequenced and analyzed 31 isolates of Paracoccidioides across South America, with particular focus on isolates from Argentina and Paraguay. The de novo sequenced isolates were compared with publicly available genomes. Phylogenetics and population genomics revealed that PCM in Argentina and Paraguay is caused by three distinct Paracoccidioides genotypes, P. brasiliensis (S1a and S1b) and P. restrepiensis (PS3). P. brasiliensis S1a isolates from Argentina are frequently associated with chronic forms of the disease. Our results suggest the existence of extensive molecular polymorphism among Paracoccidioides species, and provide a framework to begin to dissect the connection between genotypic differences in the pathogen and the clinical outcomes of the disease.

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KW - Phylogenomics

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Genomic diversity of the human pathogen Paracoccidioides across the South American continent

Abstract

Keywords

ASJC Scopus subject areas

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