@article{ed4f947f84db4833a41241e958681995,
title = "Genome-wide association identifies candidate genes for drought tolerance in coast redwood and giant sequoia",
abstract = "Drought is a major limitation for survival and growth in plants. With more frequent and severe drought episodes occurring due to climate change, it is imperative to understand the genomic and physiological basis of drought tolerance to be able to predict how species will respond in the future. In this study, univariate and multitrait multivariate genome-wide association study methods were used to identify candidate genes in two iconic and ecosystem-dominating species of the western USA, coast redwood and giant sequoia, using 10 drought-related physiological and anatomical traits and genome-wide sequence-capture single nucleotide polymorphisms. Population-level phenotypic variation was found in carbon isotope discrimination, osmotic pressure at full turgor, xylem hydraulic diameter, and total area of transporting fibers in both species. Our study identified new 78 new marker × trait associations in coast redwood and six in giant sequoia, with genes involved in a range of metabolic, stress, and signaling pathways, among other functions. This study contributes to a better understanding of the genomic basis of drought tolerance in long-generation conifers and helps guide current and future conservation efforts in the species.",
author = "{De La Torre}, {Amanda R.} and Sekhwal, {Manoj K.} and Daniela Puiu and Salzberg, {Steven L.} and Scott, {Alison D.} and Brian Allen and Neale, {David B.} and Chin, {Alana R.O.} and Buckley, {Thomas N.}",
note = "Funding Information: The authors would like to thank Emily Burns for her guidance and expertise on early stages of the project, Bill Libby for help on collection design, and Bill Werner for help with greenhouse support and propagation. TNB thanks Zane Moore for providing measurements of stomatal density in SESE, and Oliver Betz for producing micrographs of leaf cross sections in SESE and SEGI. This project was supported by a grant from Save The Redwoods League for the Redwood Genome Project (to DN). ARDLT was supported by NIFA grant ARZZ19‐0258. TNB was supported by the National Science foundation (Awards #1557906 and 1951244) and the USDA National Institute of Food and Agriculture (Hatch project 1016439 and Award no. 2020‐67013‐30913). Funding Information: The authors would like to thank Emily Burns for her guidance and expertise on early stages of the project, Bill Libby for help on collection design, and Bill Werner for help with greenhouse support and propagation. TNB thanks Zane Moore for providing measurements of stomatal density in SESE, and Oliver Betz for producing micrographs of leaf cross sections in SESE and SEGI. This project was supported by a grant from Save The Redwoods League for the Redwood Genome Project (to DN). ARDLT was supported by NIFA grant ARZZ19-0258. TNB was supported by the National Science foundation (Awards #1557906 and 1951244) and the USDA National Institute of Food and Agriculture (Hatch project 1016439 and Award no. 2020-67013-30913). Publisher Copyright: {\textcopyright} 2021 Society for Experimental Biology and John Wiley & Sons Ltd",
year = "2022",
month = jan,
doi = "10.1111/tpj.15592",
language = "English (US)",
volume = "109",
pages = "7--22",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "1",
}