Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Hossein Fazelinia; Eva Rose M. Balog; Anil Desireddy; Saumen Chakraborty; Chris J. Sheehan; Charlie E.M. Strauss; Jennifer S. Martinez

doi:10.1002/slct.201700456

Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Hossein Fazelinia, Eva Rose M. Balog, Anil Desireddy, Saumen Chakraborty, Chris J. Sheehan, Charlie E.M. Strauss, Jennifer S. Martinez

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom-up assembly of soft polypeptide-based materials. Herein, we describe the creation of a novel helix-elastin like polymer or HELP. HELP marries the specificity of coiled-coil interactions with the elasticity and stimuli-responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. The programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.

Original language	English (US)
Pages (from-to)	5008-5012
Number of pages	5
Journal	ChemistrySelect
Volume	2
Issue number	18
DOIs	https://doi.org/10.1002/slct.201700456
State	Published - Jun 21 2017
Externally published	Yes

Keywords

Elastin like polymer
Helical zipper
Protein design
Self-assembly
Stimuli responsive polymer

ASJC Scopus subject areas

General Chemistry

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10.1002/slct.201700456

Cite this

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title = "Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly",

abstract = "The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom-up assembly of soft polypeptide-based materials. Herein, we describe the creation of a novel helix-elastin like polymer or HELP. HELP marries the specificity of coiled-coil interactions with the elasticity and stimuli-responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. The programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.",

keywords = "Elastin like polymer, Helical zipper, Protein design, Self-assembly, Stimuli responsive polymer",

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language = "English (US)",

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AU - Fazelinia, Hossein

AU - Balog, Eva Rose M.

AU - Desireddy, Anil

AU - Chakraborty, Saumen

AU - Sheehan, Chris J.

AU - Strauss, Charlie E.M.

AU - Martinez, Jennifer S.

PY - 2017/6/21

Y1 - 2017/6/21

N2 - The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom-up assembly of soft polypeptide-based materials. Herein, we describe the creation of a novel helix-elastin like polymer or HELP. HELP marries the specificity of coiled-coil interactions with the elasticity and stimuli-responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. The programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.

AB - The rich palette of chemical and functional diversity found within peptides has driven the recent interest in bottom-up assembly of soft polypeptide-based materials. Herein, we describe the creation of a novel helix-elastin like polymer or HELP. HELP marries the specificity of coiled-coil interactions with the elasticity and stimuli-responsive behavior of elastin, a class of polymers with extraordinary elasticity. We used Rosetta to computationally design highly specific protein helical zippers to connect short elastin segments. The programmed pairing between the helical blocks of two individual chains produced a genetically encoded material that, when deposited on a substrate, formed a flexible and porous 2D planar network with controllable porosity that responds dynamically upon application of stimuli.

KW - Elastin like polymer

KW - Helical zipper

KW - Protein design

KW - Self-assembly

KW - Stimuli responsive polymer

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Genetically Engineered Elastomeric Polymer Network through Protein Zipper Assembly

Abstract

Keywords

ASJC Scopus subject areas

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