Foliar injury, leaf gas exchange and biomass responses of black cherry (Prunus serotina Ehrh.) half-sibling families to ozone exposure

K. B. Kouterick; J. M. Skelly; T. S. Fredericksen; K. C. Steiner; T. E. Kolb; J. A. Ferdinand

doi:10.1016/S0269-7491(99)00125-6

Foliar injury, leaf gas exchange and biomass responses of black cherry (Prunus serotina Ehrh.) half-sibling families to ozone exposure

K. B. Kouterick, J. M. Skelly, T. S. Fredericksen, K. C. Steiner, T. E. Kolb, J. A. Ferdinand

Forestry, School of

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Open pollinated families of black cherry seedlings were studied to determine genotypic differences in foliar ozone injury and leaf gas exchange in 1994 and growth response following three growing seasons. An O₃-sensitive half-sibling family (R-12) and an O₃-tolerant half-sibling family (MO-7) planted in natural soil were studied along with generic nursery stock (NS) seedlings. Ozone exposure treatments were provided through open top chambers and consisted of 50, 75, and 97% of ambient ozone, and open plots from May 9 to August 26, 1994. Ambient ozone concentrations reached an hourly peak of 88 ppb with 7-hour averages ranging from 39 to 46 ppb. Seedlings in the 50 and 75% of ambient chambers were never exposed to greater than 80 ppb O₃. Visible foliar ozone injury (stipple) was significantly higher for R-12 seedlings than MO-7 seedlings and increased with increasing ozone exposures. For the chamber treatments averaged over all families, there was no significant difference in stomatal conductance and net photosynthetic rates, but there was a significant decrease in root biomass, and a significant decrease in root/shoot ratio between the 50 and 97% of ambient chambers. Stomatal conductance and net photosynthetic rates were significantly different between families with R-12 seedlings generally greater than MO-7 seedlings. The R-12 seedlings had a 7.5 mmol m^-2 increase in ozone uptake compared to MO-7, and at the same cumulative O₃ exposure R-12 exhibited 40.9% stippled leaf area, whereas MO-7 had 9.2% stippled leaf area. Significant differences were observed in stem volume growth and total final biomass between the open-top chambers and open plots. Although R-12 had the most severe foliar ozone injury, this family had significantly greater stem volume growth and total final biomass than MO-7 and NS seedlings. Root:shoot ratio was not significantly different between MO-7 and R-12 seedlings.

Original language	English (US)
Pages (from-to)	117-126
Number of pages	10
Journal	Environmental Pollution
Volume	107
Issue number	1
DOIs	https://doi.org/10.1016/S0269-7491(99)00125-6
State	Published - Jan 2000

Keywords

Biomass
Black cherry
Ozone
Ozone uptake
Stomatal conductance

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

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10.1016/S0269-7491(99)00125-6

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@article{a766270baf94411b94199edec9d9f494,

title = "Foliar injury, leaf gas exchange and biomass responses of black cherry (Prunus serotina Ehrh.) half-sibling families to ozone exposure",

abstract = "Open pollinated families of black cherry seedlings were studied to determine genotypic differences in foliar ozone injury and leaf gas exchange in 1994 and growth response following three growing seasons. An O3-sensitive half-sibling family (R-12) and an O3-tolerant half-sibling family (MO-7) planted in natural soil were studied along with generic nursery stock (NS) seedlings. Ozone exposure treatments were provided through open top chambers and consisted of 50, 75, and 97% of ambient ozone, and open plots from May 9 to August 26, 1994. Ambient ozone concentrations reached an hourly peak of 88 ppb with 7-hour averages ranging from 39 to 46 ppb. Seedlings in the 50 and 75% of ambient chambers were never exposed to greater than 80 ppb O3. Visible foliar ozone injury (stipple) was significantly higher for R-12 seedlings than MO-7 seedlings and increased with increasing ozone exposures. For the chamber treatments averaged over all families, there was no significant difference in stomatal conductance and net photosynthetic rates, but there was a significant decrease in root biomass, and a significant decrease in root/shoot ratio between the 50 and 97% of ambient chambers. Stomatal conductance and net photosynthetic rates were significantly different between families with R-12 seedlings generally greater than MO-7 seedlings. The R-12 seedlings had a 7.5 mmol m-2 increase in ozone uptake compared to MO-7, and at the same cumulative O3 exposure R-12 exhibited 40.9% stippled leaf area, whereas MO-7 had 9.2% stippled leaf area. Significant differences were observed in stem volume growth and total final biomass between the open-top chambers and open plots. Although R-12 had the most severe foliar ozone injury, this family had significantly greater stem volume growth and total final biomass than MO-7 and NS seedlings. Root:shoot ratio was not significantly different between MO-7 and R-12 seedlings.",

keywords = "Biomass, Black cherry, Ozone, Ozone uptake, Stomatal conductance",

author = "Kouterick, {K. B.} and Skelly, {J. M.} and Fredericksen, {T. S.} and Steiner, {K. C.} and Kolb, {T. E.} and Ferdinand, {J. A.}",

note = "Funding Information: This study was funded by the United States Environmental Protection Agency cooperative agreement CR820417-01-0 with additional cooperation from the Pennsylvania Department of Conservation and Natural Resources, Bureau of Forestry Region No. 9, and the Penelec Corporation, GPU. The authors thank Paul Augustine and Lee Warren of DCNR for their support in this study, and a special thanks to Jianwei Zhang, Jim Savage, Ken Snyder, Brian Joyce, and Steve Luetke for their invaluable efforts. ",

year = "2000",

month = jan,

doi = "10.1016/S0269-7491(99)00125-6",

language = "English (US)",

volume = "107",

pages = "117--126",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier Ltd",

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T1 - Foliar injury, leaf gas exchange and biomass responses of black cherry (Prunus serotina Ehrh.) half-sibling families to ozone exposure

AU - Kouterick, K. B.

AU - Skelly, J. M.

AU - Fredericksen, T. S.

AU - Steiner, K. C.

AU - Kolb, T. E.

AU - Ferdinand, J. A.

N1 - Funding Information: This study was funded by the United States Environmental Protection Agency cooperative agreement CR820417-01-0 with additional cooperation from the Pennsylvania Department of Conservation and Natural Resources, Bureau of Forestry Region No. 9, and the Penelec Corporation, GPU. The authors thank Paul Augustine and Lee Warren of DCNR for their support in this study, and a special thanks to Jianwei Zhang, Jim Savage, Ken Snyder, Brian Joyce, and Steve Luetke for their invaluable efforts.

PY - 2000/1

Y1 - 2000/1

N2 - Open pollinated families of black cherry seedlings were studied to determine genotypic differences in foliar ozone injury and leaf gas exchange in 1994 and growth response following three growing seasons. An O3-sensitive half-sibling family (R-12) and an O3-tolerant half-sibling family (MO-7) planted in natural soil were studied along with generic nursery stock (NS) seedlings. Ozone exposure treatments were provided through open top chambers and consisted of 50, 75, and 97% of ambient ozone, and open plots from May 9 to August 26, 1994. Ambient ozone concentrations reached an hourly peak of 88 ppb with 7-hour averages ranging from 39 to 46 ppb. Seedlings in the 50 and 75% of ambient chambers were never exposed to greater than 80 ppb O3. Visible foliar ozone injury (stipple) was significantly higher for R-12 seedlings than MO-7 seedlings and increased with increasing ozone exposures. For the chamber treatments averaged over all families, there was no significant difference in stomatal conductance and net photosynthetic rates, but there was a significant decrease in root biomass, and a significant decrease in root/shoot ratio between the 50 and 97% of ambient chambers. Stomatal conductance and net photosynthetic rates were significantly different between families with R-12 seedlings generally greater than MO-7 seedlings. The R-12 seedlings had a 7.5 mmol m-2 increase in ozone uptake compared to MO-7, and at the same cumulative O3 exposure R-12 exhibited 40.9% stippled leaf area, whereas MO-7 had 9.2% stippled leaf area. Significant differences were observed in stem volume growth and total final biomass between the open-top chambers and open plots. Although R-12 had the most severe foliar ozone injury, this family had significantly greater stem volume growth and total final biomass than MO-7 and NS seedlings. Root:shoot ratio was not significantly different between MO-7 and R-12 seedlings.

AB - Open pollinated families of black cherry seedlings were studied to determine genotypic differences in foliar ozone injury and leaf gas exchange in 1994 and growth response following three growing seasons. An O3-sensitive half-sibling family (R-12) and an O3-tolerant half-sibling family (MO-7) planted in natural soil were studied along with generic nursery stock (NS) seedlings. Ozone exposure treatments were provided through open top chambers and consisted of 50, 75, and 97% of ambient ozone, and open plots from May 9 to August 26, 1994. Ambient ozone concentrations reached an hourly peak of 88 ppb with 7-hour averages ranging from 39 to 46 ppb. Seedlings in the 50 and 75% of ambient chambers were never exposed to greater than 80 ppb O3. Visible foliar ozone injury (stipple) was significantly higher for R-12 seedlings than MO-7 seedlings and increased with increasing ozone exposures. For the chamber treatments averaged over all families, there was no significant difference in stomatal conductance and net photosynthetic rates, but there was a significant decrease in root biomass, and a significant decrease in root/shoot ratio between the 50 and 97% of ambient chambers. Stomatal conductance and net photosynthetic rates were significantly different between families with R-12 seedlings generally greater than MO-7 seedlings. The R-12 seedlings had a 7.5 mmol m-2 increase in ozone uptake compared to MO-7, and at the same cumulative O3 exposure R-12 exhibited 40.9% stippled leaf area, whereas MO-7 had 9.2% stippled leaf area. Significant differences were observed in stem volume growth and total final biomass between the open-top chambers and open plots. Although R-12 had the most severe foliar ozone injury, this family had significantly greater stem volume growth and total final biomass than MO-7 and NS seedlings. Root:shoot ratio was not significantly different between MO-7 and R-12 seedlings.

KW - Biomass

KW - Black cherry

KW - Ozone

KW - Ozone uptake

KW - Stomatal conductance

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Foliar injury, leaf gas exchange and biomass responses of black cherry (Prunus serotina Ehrh.) half-sibling families to ozone exposure

Abstract

Keywords

ASJC Scopus subject areas

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