TY - JOUR
T1 - First report and whole-genome sequencing of Pseudochrobactrum saccharolyticum in Latin America
AU - Pereira, Carine Rodrigues
AU - de Jesus Sousa, Thiago
AU - Lima da Silva, Alessandra
AU - Gonçalves dos Santos, Roselane
AU - Minharro, Sílvia
AU - Costa Custódio, Dirceia Aparecida
AU - Pickard, Derek J.
AU - O'Callaghan, David
AU - Foster, Jeffrey T.
AU - de Castro Soares, Siomar
AU - Juca Ramos, Rommel Thiago
AU - Góes-Neto, Aristóteles
AU - Matiuzzi da Costa, Mateus
AU - Lage, Andrey Pereira
AU - Azevedo, Vasco
AU - Seles Dorneles, Elaine Maria
N1 - Publisher Copyright:
© 2022 Institut Pasteur
PY - 2023/1/1
Y1 - 2023/1/1
N2 - The Brucellaceae family comprises microorganisms similar both phenotypically and genotypically, making it difficult to identify the etiological agent of these infections. This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. Strain 115 was isolated in 2007 from a bovine in Brazil and was initially classified as Brucella spp. by classical microbiological tests and bcsp31 PCR. The antimicrobial susceptibility of strain 115 was tested against drugs used to treat human brucellosis by minimal inhibitory concentration test. Subsequently, the whole genome of the strain was sequenced, assembled, and characterized. Phylogenetic trees built from 16S rRNA and recA gene sequences enabled the classification of strain 115 as Pseudochrobactrum spp. Phylogenomic analysis using Single Nucleotide Polymorphisms and Average Nucleotide Identity allowed the classification of the strain as P. saccharolyticum. Additionally, a Tetra Correlation Search identified one related genome from the same species, which was compared with strain 115 by analyzing genomic islands. This is the first identification and whole-genome sequence of P. saccharolyticum in Latin America and highlights a challenge in the diagnosis of bovine brucellosis, which could be solved by including the sequencing of 16S rRNA and recA genes in routine diagnostics.
AB - The Brucellaceae family comprises microorganisms similar both phenotypically and genotypically, making it difficult to identify the etiological agent of these infections. This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. Strain 115 was isolated in 2007 from a bovine in Brazil and was initially classified as Brucella spp. by classical microbiological tests and bcsp31 PCR. The antimicrobial susceptibility of strain 115 was tested against drugs used to treat human brucellosis by minimal inhibitory concentration test. Subsequently, the whole genome of the strain was sequenced, assembled, and characterized. Phylogenetic trees built from 16S rRNA and recA gene sequences enabled the classification of strain 115 as Pseudochrobactrum spp. Phylogenomic analysis using Single Nucleotide Polymorphisms and Average Nucleotide Identity allowed the classification of the strain as P. saccharolyticum. Additionally, a Tetra Correlation Search identified one related genome from the same species, which was compared with strain 115 by analyzing genomic islands. This is the first identification and whole-genome sequence of P. saccharolyticum in Latin America and highlights a challenge in the diagnosis of bovine brucellosis, which could be solved by including the sequencing of 16S rRNA and recA genes in routine diagnostics.
KW - Antimicrobial resistance
KW - Brucellaceae
KW - Phylogenetic
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U2 - 10.1016/j.micinf.2022.105018
DO - 10.1016/j.micinf.2022.105018
M3 - Article
C2 - 35940401
AN - SCOPUS:85143662215
SN - 1286-4579
VL - 25
JO - Microbes and Infection
JF - Microbes and Infection
IS - 1-2
M1 - 105018
ER -