Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation

Katherine S. Lovejoy; Lauren E. Davis; Lisa M. McClellan; Antonietta M. Lillo; John D. Welsh; Emily N. Schmidt; Claire K. Sanders; Alexander J. Lou; David T. Fox; Andrew T. Koppisch; Rico E. Del Sesto

doi:10.1007/s10811-012-9907-0

Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation

Katherine S. Lovejoy
, Lauren E. Davis
, Lisa M. McClellan
, Antonietta M. Lillo
, John D. Welsh
, Emily N. Schmidt
, Claire K. Sanders
, Alexander J. Lou
, David T. Fox
, Andrew T. Koppisch
, Rico E. Del Sesto

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our results suggest that ionic liquids have the potential to serve as novel biocompatible milking agents for extracting high-value chemicals from the microbes, with toxicity to both species minimized by considerations of ionic liquid structure and hydrophobicity.

Original language	English (US)
Pages (from-to)	973-981
Number of pages	9
Journal	Journal of Applied Phycology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1007/s10811-012-9907-0
State	Published - Aug 2013

Keywords

Biofuels
Botryococcenes
Ionic liquids
Milking

ASJC Scopus subject areas

Aquatic Science
Plant Science

Access to Document

10.1007/s10811-012-9907-0

Cite this

@article{597d3399fe5348f9b4885cc9b1eea6cd,

title = "Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation",

abstract = "Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our results suggest that ionic liquids have the potential to serve as novel biocompatible milking agents for extracting high-value chemicals from the microbes, with toxicity to both species minimized by considerations of ionic liquid structure and hydrophobicity.",

keywords = "Biofuels, Botryococcenes, Ionic liquids, Milking",

author = "Lovejoy, \{Katherine S.\} and Davis, \{Lauren E.\} and McClellan, \{Lisa M.\} and Lillo, \{Antonietta M.\} and Welsh, \{John D.\} and Schmidt, \{Emily N.\} and Sanders, \{Claire K.\} and Lou, \{Alexander J.\} and Fox, \{David T.\} and Koppisch, \{Andrew T.\} and \{Del Sesto\}, \{Rico E.\}",

note = "Funding Information: Acknowledgments This work was supported by the US Department of Energy LANL LDRD Program, National Alliance for Advanced Biofuels and Bioproducts, and the Los Alamos National Flow Cytometry Resource funded by the National Center for Research Resources of NIH (grant P41-RR01315). The authors are grateful to Cytec Canada for supplying the [R3R′P]Cl starting material and to Taylor Weiss and Dr. Tim Devarenne (Texas A\&M University) for supplying an authentic sample of isolated botryococcenes. We also thank Dr. Taraka Dale (LANL) for the critical analysis of the flow cytometry data. Los Alamos National Laboratory is operated by Los Alamos National Security, LLC, for the National Nuclear Security Administration of the US Department of Energy under contract DE-AC52-06NA25396.",

year = "2013",

month = aug,

doi = "10.1007/s10811-012-9907-0",

language = "English (US)",

volume = "25",

pages = "973--981",

journal = "Journal of Applied Phycology",

issn = "0921-8971",

publisher = "Springer Science and Business Media B.V.",

number = "4",

}

TY - JOUR

T1 - Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation

AU - Lovejoy, Katherine S.

AU - Davis, Lauren E.

AU - McClellan, Lisa M.

AU - Lillo, Antonietta M.

AU - Welsh, John D.

AU - Schmidt, Emily N.

AU - Sanders, Claire K.

AU - Lou, Alexander J.

AU - Fox, David T.

AU - Koppisch, Andrew T.

AU - Del Sesto, Rico E.

N1 - Funding Information: Acknowledgments This work was supported by the US Department of Energy LANL LDRD Program, National Alliance for Advanced Biofuels and Bioproducts, and the Los Alamos National Flow Cytometry Resource funded by the National Center for Research Resources of NIH (grant P41-RR01315). The authors are grateful to Cytec Canada for supplying the [R3R′P]Cl starting material and to Taylor Weiss and Dr. Tim Devarenne (Texas A&M University) for supplying an authentic sample of isolated botryococcenes. We also thank Dr. Taraka Dale (LANL) for the critical analysis of the flow cytometry data. Los Alamos National Laboratory is operated by Los Alamos National Security, LLC, for the National Nuclear Security Administration of the US Department of Energy under contract DE-AC52-06NA25396.

PY - 2013/8

Y1 - 2013/8

N2 - Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our results suggest that ionic liquids have the potential to serve as novel biocompatible milking agents for extracting high-value chemicals from the microbes, with toxicity to both species minimized by considerations of ionic liquid structure and hydrophobicity.

AB - Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our results suggest that ionic liquids have the potential to serve as novel biocompatible milking agents for extracting high-value chemicals from the microbes, with toxicity to both species minimized by considerations of ionic liquid structure and hydrophobicity.

KW - Biofuels

KW - Botryococcenes

KW - Ionic liquids

KW - Milking

UR - https://www.scopus.com/pages/publications/84879952721

UR - https://www.scopus.com/pages/publications/84879952721#tab=citedBy

U2 - 10.1007/s10811-012-9907-0

DO - 10.1007/s10811-012-9907-0

M3 - Article

AN - SCOPUS:84879952721

SN - 0921-8971

VL - 25

SP - 973

EP - 981

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

IS - 4

ER -

Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this