@article{597d3399fe5348f9b4885cc9b1eea6cd,
title = "Evaluation of ionic liquids on phototrophic microbes and their use in biofuel extraction and isolation",
abstract = "Multiple ionic liquids (ILs) were assessed for their ability to extract branched, unsaturated hydrocarbons from an aqueous medium. In addition, IL cytotoxicity studies were performed on two phototrophic microbes, Synechocystis sp. PCC6803 and Botryococcus braunii var Showa. The optimum IL for use in an isoprenoid hydrocarbon extraction may vary based on the biological source of the isoprenoids. Our results suggest that ionic liquids have the potential to serve as novel biocompatible milking agents for extracting high-value chemicals from the microbes, with toxicity to both species minimized by considerations of ionic liquid structure and hydrophobicity.",
keywords = "Biofuels, Botryococcenes, Ionic liquids, Milking",
author = "Lovejoy, {Katherine S.} and Davis, {Lauren E.} and McClellan, {Lisa M.} and Lillo, {Antonietta M.} and Welsh, {John D.} and Schmidt, {Emily N.} and Sanders, {Claire K.} and Lou, {Alexander J.} and Fox, {David T.} and Koppisch, {Andrew T.} and {Del Sesto}, {Rico E.}",
note = "Funding Information: Acknowledgments This work was supported by the US Department of Energy LANL LDRD Program, National Alliance for Advanced Biofuels and Bioproducts, and the Los Alamos National Flow Cytometry Resource funded by the National Center for Research Resources of NIH (grant P41-RR01315). The authors are grateful to Cytec Canada for supplying the [R3R′P]Cl starting material and to Taylor Weiss and Dr. Tim Devarenne (Texas A&M University) for supplying an authentic sample of isolated botryococcenes. We also thank Dr. Taraka Dale (LANL) for the critical analysis of the flow cytometry data. Los Alamos National Laboratory is operated by Los Alamos National Security, LLC, for the National Nuclear Security Administration of the US Department of Energy under contract DE-AC52-06NA25396.",
year = "2013",
month = aug,
doi = "10.1007/s10811-012-9907-0",
language = "English (US)",
volume = "25",
pages = "973--981",
journal = "Journal of Applied Phycology",
issn = "0921-8971",
publisher = "Springer Netherlands",
number = "4",
}