Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

Research output: Contribution to journalArticlepeer-review

335 Scopus citations

Abstract

Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
JournalGene
Volume97
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

Keywords

  • broad-host-range expression vectors
  • DNA sequencing
  • multiple cloning site
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Escherichia-Pseudomonas shuttle vectors derived from pUC18/19'. Together they form a unique fingerprint.

Cite this