Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

H. P. Schweizer

doi:10.1016/0378-1119(91)90016-5

Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

H. P. Schweizer

Research output: Contribution to journal › Article › peer-review

347 Scopus citations

Abstract

Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

Original language	English (US)
Pages (from-to)	109-112
Number of pages	4
Journal	Gene
Volume	97
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90016-5
State	Published - 1991
Externally published	Yes

Keywords

DNA sequencing
Recombinant DNA
broad-host-range expression vectors
multiple cloning site

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90016-5

Cite this

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title = "Escherichia-Pseudomonas shuttle vectors derived from pUC18/19",

abstract = "Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.",

keywords = "DNA sequencing, Recombinant DNA, broad-host-range expression vectors, multiple cloning site",

author = "Schweizer, {H. P.}",

note = "Funding Information: I thank A. Schryvers for generous support and D. Storey for the gift of pUC 181 .8D NA. This researchw as supported by a grant from the Canadian Cystic Fibrosis Foundation administeredb y D.E. Woods and by grants from the Medical Research Council of Canada and the University of Calgary. H.P.S. is a MRC Medical Scholar.",

year = "1991",

doi = "10.1016/0378-1119(91)90016-5",

language = "English (US)",

volume = "97",

pages = "109--112",

journal = "Gene",

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T1 - Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

AU - Schweizer, H. P.

N1 - Funding Information: I thank A. Schryvers for generous support and D. Storey for the gift of pUC 181 .8D NA. This researchw as supported by a grant from the Canadian Cystic Fibrosis Foundation administeredb y D.E. Woods and by grants from the Medical Research Council of Canada and the University of Calgary. H.P.S. is a MRC Medical Scholar.

PY - 1991

Y1 - 1991

N2 - Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

AB - Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

KW - DNA sequencing

KW - Recombinant DNA

KW - broad-host-range expression vectors

KW - multiple cloning site

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JO - Gene

JF - Gene

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Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

Abstract

Keywords

ASJC Scopus subject areas

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