Escherichia-Pseudomonas shuttle vectors derived from pUC18/19

Research output: Contribution to journalArticlepeer-review

347 Scopus citations


Two new broad-host-range plasmid vectors, pUCPIS and pUCP19, which are stably maintained in Escherichia coli and Pseudomonas aeruginosa have been constructed. The plasmids are based on the E. coli pUC18 and pUC19 vectors and possess all their features: (i) convenient direct screening of recombinants; (ii) versatile multiple cloning site; (iii) use as sequencing and expression vectors; (iv) small size; and (v) intermediate to high copy number.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
Issue number1
StatePublished - 1991
Externally publishedYes


  • DNA sequencing
  • Recombinant DNA
  • broad-host-range expression vectors
  • multiple cloning site

ASJC Scopus subject areas

  • Genetics


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