Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus

Melissa Birkett, Katherine V. Fite

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Serotonin (5-HT) immunostained sections were analyzed using integrated optical density (IOD) measures obtained throughout the dorsal raphe nucleus (DRN) in Mongolian gerbils at selected times during a 12:12 h light:dark cycle. Substantial diurnal variation occurred in 5-HT neuronal staining density, with lowest and highest IOD values occurring at the light/dark and dark/light transitions, respectively. The injection of pargyline and tryptophan increased 5-HT immunostaining comparable to the highest level observed in control animals. Transitions between light and dark periods appear to be major environmental events that influence 5-HT levels in the DRN.

Original languageEnglish (US)
Pages (from-to)180-184
Number of pages5
JournalBrain Research
Issue number1-2
StatePublished - Feb 9 2005


  • 5-HT immunocytochemistry
  • Mongolian gerbil
  • Optical density analysis

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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