Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus

Melissa Birkett; Katherine V. Fite

doi:10.1016/j.brainres.2004.11.053

Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus

Melissa Birkett, Katherine V. Fite

Psychological Sciences

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Serotonin (5-HT) immunostained sections were analyzed using integrated optical density (IOD) measures obtained throughout the dorsal raphe nucleus (DRN) in Mongolian gerbils at selected times during a 12:12 h light:dark cycle. Substantial diurnal variation occurred in 5-HT neuronal staining density, with lowest and highest IOD values occurring at the light/dark and dark/light transitions, respectively. The injection of pargyline and tryptophan increased 5-HT immunostaining comparable to the highest level observed in control animals. Transitions between light and dark periods appear to be major environmental events that influence 5-HT levels in the DRN.

Original language	English (US)
Pages (from-to)	180-184
Number of pages	5
Journal	Brain Research
Volume	1034
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2004.11.053
State	Published - Feb 9 2005

Keywords

5-HT immunocytochemistry
Mongolian gerbil
Optical density analysis

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2004.11.053

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title = "Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus",

abstract = "Serotonin (5-HT) immunostained sections were analyzed using integrated optical density (IOD) measures obtained throughout the dorsal raphe nucleus (DRN) in Mongolian gerbils at selected times during a 12:12 h light:dark cycle. Substantial diurnal variation occurred in 5-HT neuronal staining density, with lowest and highest IOD values occurring at the light/dark and dark/light transitions, respectively. The injection of pargyline and tryptophan increased 5-HT immunostaining comparable to the highest level observed in control animals. Transitions between light and dark periods appear to be major environmental events that influence 5-HT levels in the DRN.",

keywords = "5-HT immunocytochemistry, Mongolian gerbil, Optical density analysis",

author = "Melissa Birkett and Fite, {Katherine V.}",

note = "Funding Information: This research was supported by NSF IBN-0091660. We thank Lynn Bengston for her excellent technical assistance.",

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T1 - Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus

AU - Birkett, Melissa

AU - Fite, Katherine V.

N1 - Funding Information: This research was supported by NSF IBN-0091660. We thank Lynn Bengston for her excellent technical assistance.

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AB - Serotonin (5-HT) immunostained sections were analyzed using integrated optical density (IOD) measures obtained throughout the dorsal raphe nucleus (DRN) in Mongolian gerbils at selected times during a 12:12 h light:dark cycle. Substantial diurnal variation occurred in 5-HT neuronal staining density, with lowest and highest IOD values occurring at the light/dark and dark/light transitions, respectively. The injection of pargyline and tryptophan increased 5-HT immunostaining comparable to the highest level observed in control animals. Transitions between light and dark periods appear to be major environmental events that influence 5-HT levels in the DRN.

KW - 5-HT immunocytochemistry

KW - Mongolian gerbil

KW - Optical density analysis

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Diurnal variation in serotonin immunoreactivity in the dorsal raphe nucleus

Abstract

Keywords

ASJC Scopus subject areas

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