Distribution of nucleosides in populations of Cordyceps cicadae

Wen Bo Zeng, Hong Yu, Feng Ge, Jun Yuan Yang, Zi Hong Chen, Yuan Bing Wang, Yong Dong Dai, Alison Adams

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


A rapid HPLC method had been developed and used for the simultaneous determination of 10 nucleosides (uracil, uridine, 2'-deoxyuridine, inosine, guanosine, thymidine, adenine, adenosine, 2'-deoxyadenosine and cordycepin) in 10 populations of Cordyceps cicadae, in order to compare four populations of Ophicordyceps sinensis and one population of Cordyceps militaris. Statistical analysis system (SAS) 8.1 was used to analyze the nucleoside data. The pattern of nucleoside distribution was analyzed in the sampled populations of C. cicadae, O. sinensis and C. militaris, using descriptive statistical analysis, nested analysis and Q cluster analysis. The total amount of the 10 nucleosides in coremium was 1,463.89-5,678.21 μg/g in 10 populations of C. cicadae, 1,369.80-3,941.64 μg/g in sclerotium. The average contents of the 10 analytes were 4,392.37 μg/g and 3,016.06 μg/g in coremium and sclerotium, respectively. The coefficient of variation (CV) of nucleosides ranged from 8.36% to 112.36% in coremium of C. cicadae, and from 10.77% to 155.87% in sclerotium of C. cicadae. The CV of the nucleosides was wide within C. cicadae populations. The nested variation analysis by the nine nucleosides' distribution indicated that about 42.29% of the nucleoside variability in coremium was attributable to the differentiation among populations, and the remaining 57.71% resided in the populations. It was also shown that about 28.94% of the variation in sclerotium was expressed between populations, while most of the variation (71.06%) corresponded to the populations.

Original languageEnglish (US)
Pages (from-to)6123-6141
Number of pages19
Issue number5
StatePublished - May 2014


  • Cordyceps cicadae
  • Distribution
  • Nucleosides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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