TY - JOUR
T1 - Developmental timing of perchlorate exposure alters threespine stickleback dermal bone
AU - Furin, Christoff G.
AU - von Hippel, Frank A.
AU - Postlethwait, John
AU - Buck, C. Loren
AU - Cresko, William A.
AU - O'Hara, Todd M.
N1 - Funding Information:
The authors thank T. Villafranca, M. Sherbick, L. Smayda, E. Kittel, and L. Matthews for laboratory support. Thanks to R. Bernhardt and J. Willacker for advice and discussion. Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number P20GM103395 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding was also provided by NIH Grant Number 1R01ES017039 . Fish were collected under Alaska Department of Fish and Game permit SF-2008-019, and all research protocols were approved by the UAA Institutional Animal Care and Use Committee (protocol 2007vonhi1).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Adequate levels of thyroid hormone are critical during development and metamorphosis, and for maintaining metabolic homeostasis. Perchlorate, a common contaminant of water sources, inhibits thyroid function in vertebrates. We utilized threespine stickleback (Gasterosteus aculeatus) to determine if timing of perchlorate exposure during development impacts adult dermal skeletal phenotypes. Fish were exposed to water contaminated with perchlorate (30mg/L or 100mg/L) beginning at 0, 3, 7, 14, 21, 42, 154 or 305. days post fertilization until sexual maturity at 1. year of age. A reciprocal treatment moved stickleback from contaminated to clean water on the same schedule providing for different stages of initial exposure and different treatment durations. Perchlorate exposure caused concentration-dependent significant differences in growth for some bony traits. Continuous exposure initiated within the first 21. days post fertilization had the greatest effects on skeletal traits. Exposure to perchlorate at this early stage can result in small traits or abnormal skeletal morphology of adult fish which could affect predator avoidance and survival.
AB - Adequate levels of thyroid hormone are critical during development and metamorphosis, and for maintaining metabolic homeostasis. Perchlorate, a common contaminant of water sources, inhibits thyroid function in vertebrates. We utilized threespine stickleback (Gasterosteus aculeatus) to determine if timing of perchlorate exposure during development impacts adult dermal skeletal phenotypes. Fish were exposed to water contaminated with perchlorate (30mg/L or 100mg/L) beginning at 0, 3, 7, 14, 21, 42, 154 or 305. days post fertilization until sexual maturity at 1. year of age. A reciprocal treatment moved stickleback from contaminated to clean water on the same schedule providing for different stages of initial exposure and different treatment durations. Perchlorate exposure caused concentration-dependent significant differences in growth for some bony traits. Continuous exposure initiated within the first 21. days post fertilization had the greatest effects on skeletal traits. Exposure to perchlorate at this early stage can result in small traits or abnormal skeletal morphology of adult fish which could affect predator avoidance and survival.
KW - Endocrine disruption
KW - Gasterosteus aculeatus
KW - Hypothyroidism
KW - Skeletal abnormality
KW - Thyroid hormone
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U2 - 10.1016/j.ygcen.2015.02.016
DO - 10.1016/j.ygcen.2015.02.016
M3 - Article
C2 - 25753171
AN - SCOPUS:84939415946
SN - 0016-6480
VL - 219
SP - 36
EP - 44
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
ER -