Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

Joseph D. Busch; Russell Benford; Talima Pearson; Eldon Palmer; Russell P. Balda; Paul Keim

doi:10.1007/s10592-008-9616-z

Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

Joseph D. Busch, Russell Benford, Talima Pearson, Eldon Palmer, Russell P. Balda, Paul Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H _O 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.

Original language	English (US)
Pages (from-to)	689-691
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/s10592-008-9616-z
State	Published - Jun 2009

Keywords

Conservation
Gymnorhinus cyanocephalus
Hybridization enrichment
Pinyon pine
Tetranucleotide

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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title = "Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)",

abstract = "The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H O 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.",

keywords = "Conservation, Gymnorhinus cyanocephalus, Hybridization enrichment, Pinyon pine, Tetranucleotide",

author = "Busch, {Joseph D.} and Russell Benford and Talima Pearson and Eldon Palmer and Balda, {Russell P.} and Paul Keim",

note = "Funding Information: Acknowledgements We thank Dr. Shou-Hsien Li for advice on hybridization enrichment. Critical support for specimen collection and data analysis was provided by members of the Northern Arizona University Avian Cognition Laboratory and Keim Genetics Lab. Financial support was provided by National Science Foundation Grant #9982883 and Northern Arizona University. All field procedures were approved by the Northern Arizona University Institutional Animal Care and Use Committee. Additional information about permits and protocols is available upon request.",

year = "2009",

month = jun,

doi = "10.1007/s10592-008-9616-z",

language = "English (US)",

volume = "10",

pages = "689--691",

journal = "Conservation Genetics",

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publisher = "Springer Netherlands",

number = "3",

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T1 - Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

AU - Busch, Joseph D.

AU - Benford, Russell

AU - Pearson, Talima

AU - Palmer, Eldon

AU - Balda, Russell P.

AU - Keim, Paul

N1 - Funding Information: Acknowledgements We thank Dr. Shou-Hsien Li for advice on hybridization enrichment. Critical support for specimen collection and data analysis was provided by members of the Northern Arizona University Avian Cognition Laboratory and Keim Genetics Lab. Financial support was provided by National Science Foundation Grant #9982883 and Northern Arizona University. All field procedures were approved by the Northern Arizona University Institutional Animal Care and Use Committee. Additional information about permits and protocols is available upon request.

PY - 2009/6

Y1 - 2009/6

N2 - The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H O 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.

AB - The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H O 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.

KW - Conservation

KW - Gymnorhinus cyanocephalus

KW - Hybridization enrichment

KW - Pinyon pine

KW - Tetranucleotide

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Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

Abstract

Keywords

ASJC Scopus subject areas

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