Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

Kelly W. Sheff; Emily R. York; Elizabeth M. Driebe; Bridget M. Barker; Steven D. Rounsley; Victor G. Waddell; Stephen M. Beckstrom-Sternberg; James S. Beckstrom-Sternberg; Paul S. Keim; David M. Engelthaler

doi:10.3109/13693780903218990

Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

Kelly W. Sheff
, Emily R. York
, Elizabeth M. Driebe
, Bridget M. Barker
, Steven D. Rounsley
, Victor G. Waddell
, Stephen M. Beckstrom-Sternberg
, James S. Beckstrom-Sternberg
, Paul S. Keim
, David M. Engelthaler

Biological Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Original language	English (US)
Pages (from-to)	466-469
Number of pages	4
Journal	Medical Mycology
Volume	48
Issue number	3
DOIs	https://doi.org/10.3109/13693780903218990
State	Published - 2010

Keywords

Coccidioides
Coccidioidomycosis
Epidemiology

ASJC Scopus subject areas

Infectious Diseases

Access to Document

10.3109/13693780903218990

Cite this

Sheff, K. W., York, E. R., Driebe, E. M., Barker, B. M., Rounsley, S. D., Waddell, V. G., Beckstrom-Sternberg, S. M., Beckstrom-Sternberg, J. S., Keim, P. S., & Engelthaler, D. M. (2010). Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii. Medical Mycology, 48(3), 466-469. https://doi.org/10.3109/13693780903218990

Sheff, KW, York, ER, Driebe, EM, Barker, BM, Rounsley, SD, Waddell, VG, Beckstrom-Sternberg, SM, Beckstrom-Sternberg, JS, Keim, PS & Engelthaler, DM 2010, 'Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii', Medical Mycology, vol. 48, no. 3, pp. 466-469. https://doi.org/10.3109/13693780903218990

@article{0ab3acd8e41040ff997fe117b809bd54,

title = "Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii",

abstract = "Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.",

keywords = "Coccidioides, Coccidioidomycosis, Epidemiology",

author = "Sheff, \{Kelly W.\} and York, \{Emily R.\} and Driebe, \{Elizabeth M.\} and Barker, \{Bridget M.\} and Rounsley, \{Steven D.\} and Waddell, \{Victor G.\} and Beckstrom-Sternberg, \{Stephen M.\} and Beckstrom-Sternberg, \{James S.\} and Keim, \{Paul S.\} and Engelthaler, \{David M.\}",

note = "Funding Information: This research was supported by grants from the Arizona Biomedical Research Commission (\# 0816), Science Foundation Arizona (\# CAA0242-08) and the Helios Education Foundation.",

year = "2010",

doi = "10.3109/13693780903218990",

language = "English (US)",

volume = "48",

pages = "466--469",

journal = "Medical Mycology",

issn = "1369-3786",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

AU - Sheff, Kelly W.

AU - York, Emily R.

AU - Driebe, Elizabeth M.

AU - Barker, Bridget M.

AU - Rounsley, Steven D.

AU - Waddell, Victor G.

AU - Beckstrom-Sternberg, Stephen M.

AU - Beckstrom-Sternberg, James S.

AU - Keim, Paul S.

AU - Engelthaler, David M.

N1 - Funding Information: This research was supported by grants from the Arizona Biomedical Research Commission (# 0816), Science Foundation Arizona (# CAA0242-08) and the Helios Education Foundation.

PY - 2010

Y1 - 2010

N2 - Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

AB - Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

KW - Coccidioides

KW - Coccidioidomycosis

KW - Epidemiology

UR - https://www.scopus.com/pages/publications/77950556060

UR - https://www.scopus.com/pages/publications/77950556060#tab=citedBy

U2 - 10.3109/13693780903218990

DO - 10.3109/13693780903218990

M3 - Article

C2 - 20370360

AN - SCOPUS:77950556060

SN - 1369-3786

VL - 48

SP - 466

EP - 469

JO - Medical Mycology

JF - Medical Mycology

IS - 3

ER -

Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this