Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy

A. C. Hernández-Arteaga; H. J. Ojeda-Galván; M. C. Rodríguez-Aranda; J. F. Toro-Vázquez; J. Sánchez; M. José-Yacamán; H. R. Navarro-Contreras

doi:10.1016/j.saa.2021.120269

Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy

A. C. Hernández-Arteaga, H. J. Ojeda-Galván, M. C. Rodríguez-Aranda, J. F. Toro-Vázquez, J. Sánchez, M. José-Yacamán, H. R. Navarro-Contreras

Applied Physics and Materials Science

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In the present work the temperature response of the constitutive S1 segment of the SARS-CoV-2 Spike Glycoprotein (GPS) has been studied. The intensity of the Raman bands remained almost constant before reaching a temperature of 133 °C. At this temperature a significant reduction of peak intensities was observed. Above 144 °C the spectra ceased to show any recognizable feature as that of the GPS S1, indicating that it had transformed after the denaturation process that it was subjected. The GPS S1 change is irreversible. Hence, Raman Spectroscopy (RS) provides a precision method to determine the denaturation temperature (T_D) of dry powder GPS S1. The ability of RS was calibrated through the reproduction of T_D of other well studied proteins as well as those of the decomposition temperature of some amino acids (AA). Through this study we established a T_D of 139 ± 3 °C for powder GPS S1 of SARS-CoV-2.

Original language	English (US)
Article number	120269
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	264
DOIs	https://doi.org/10.1016/j.saa.2021.120269
State	Published - Jan 5 2022

Keywords

Denaturation temperature
Glycoprotein Spike
S1 subunit
SARS-CoV-2 virus

ASJC Scopus subject areas

Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Spectroscopy

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10.1016/j.saa.2021.120269

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Hernández-Arteaga, A. C., Ojeda-Galván, H. J., Rodríguez-Aranda, M. C., Toro-Vázquez, J. F., Sánchez, J., José-Yacamán, M., & Navarro-Contreras, H. R. (2022). Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 264, Article 120269. https://doi.org/10.1016/j.saa.2021.120269

Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy. / Hernández-Arteaga, A. C.; Ojeda-Galván, H. J.; Rodríguez-Aranda, M. C. et al.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 264, 120269, 05.01.2022.

Research output: Contribution to journal › Article › peer-review

Hernández-Arteaga, AC, Ojeda-Galván, HJ, Rodríguez-Aranda, MC, Toro-Vázquez, JF, Sánchez, J, José-Yacamán, M & Navarro-Contreras, HR 2022, 'Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy', Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 264, 120269. https://doi.org/10.1016/j.saa.2021.120269

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abstract = "In the present work the temperature response of the constitutive S1 segment of the SARS-CoV-2 Spike Glycoprotein (GPS) has been studied. The intensity of the Raman bands remained almost constant before reaching a temperature of 133 °C. At this temperature a significant reduction of peak intensities was observed. Above 144 °C the spectra ceased to show any recognizable feature as that of the GPS S1, indicating that it had transformed after the denaturation process that it was subjected. The GPS S1 change is irreversible. Hence, Raman Spectroscopy (RS) provides a precision method to determine the denaturation temperature (TD) of dry powder GPS S1. The ability of RS was calibrated through the reproduction of TD of other well studied proteins as well as those of the decomposition temperature of some amino acids (AA). Through this study we established a TD of 139 ± 3 °C for powder GPS S1 of SARS-CoV-2.",

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doi = "10.1016/j.saa.2021.120269",

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AU - Hernández-Arteaga, A. C.

AU - Ojeda-Galván, H. J.

AU - Rodríguez-Aranda, M. C.

AU - Toro-Vázquez, J. F.

AU - Sánchez, J.

AU - José-Yacamán, M.

AU - Navarro-Contreras, H. R.

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N2 - In the present work the temperature response of the constitutive S1 segment of the SARS-CoV-2 Spike Glycoprotein (GPS) has been studied. The intensity of the Raman bands remained almost constant before reaching a temperature of 133 °C. At this temperature a significant reduction of peak intensities was observed. Above 144 °C the spectra ceased to show any recognizable feature as that of the GPS S1, indicating that it had transformed after the denaturation process that it was subjected. The GPS S1 change is irreversible. Hence, Raman Spectroscopy (RS) provides a precision method to determine the denaturation temperature (TD) of dry powder GPS S1. The ability of RS was calibrated through the reproduction of TD of other well studied proteins as well as those of the decomposition temperature of some amino acids (AA). Through this study we established a TD of 139 ± 3 °C for powder GPS S1 of SARS-CoV-2.

AB - In the present work the temperature response of the constitutive S1 segment of the SARS-CoV-2 Spike Glycoprotein (GPS) has been studied. The intensity of the Raman bands remained almost constant before reaching a temperature of 133 °C. At this temperature a significant reduction of peak intensities was observed. Above 144 °C the spectra ceased to show any recognizable feature as that of the GPS S1, indicating that it had transformed after the denaturation process that it was subjected. The GPS S1 change is irreversible. Hence, Raman Spectroscopy (RS) provides a precision method to determine the denaturation temperature (TD) of dry powder GPS S1. The ability of RS was calibrated through the reproduction of TD of other well studied proteins as well as those of the decomposition temperature of some amino acids (AA). Through this study we established a TD of 139 ± 3 °C for powder GPS S1 of SARS-CoV-2.

KW - Denaturation temperature

KW - Glycoprotein Spike

KW - S1 subunit

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Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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