TY - JOUR
T1 - Determination of sialic acid levels by using surface-enhanced Raman spectroscopy in periodontitis and gingivitis
AU - Hernández-Cedillo, Alondra
AU - García-Valdivieso, Ma Guadalupe
AU - Hernández-Arteaga, Aida Catalina
AU - Patiño-Marín, Nuria
AU - Vértiz-Hernández, Ángel Antonio
AU - José-Yacamán, Miguel
AU - Navarro-Contreras, Hugo Ricardo
N1 - Publisher Copyright:
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Objectives: To compare the sialic acid (SA) levels in saliva among periodontitis-affected, gingivitis and control patients. Methods: The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface-enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA. Results: The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively. Conclusions: Our measurements by SERS corroborate that in periodontitis-affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.
AB - Objectives: To compare the sialic acid (SA) levels in saliva among periodontitis-affected, gingivitis and control patients. Methods: The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface-enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA. Results: The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively. Conclusions: Our measurements by SERS corroborate that in periodontitis-affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.
KW - Ag nanoparticles
KW - gingivitis
KW - periodontitis
KW - sialic acid
KW - surface-enhanced Raman
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U2 - 10.1111/odi.13141
DO - 10.1111/odi.13141
M3 - Article
C2 - 31177594
AN - SCOPUS:85068529135
SN - 1354-523X
VL - 25
SP - 1627
EP - 1633
JO - Oral Diseases
JF - Oral Diseases
IS - 6
ER -