TY - JOUR
T1 - Deformation measurements of neuronal excitability using incoherent holography lattice light-sheet microscope (Ihlls)
AU - Potcoava, Mariana
AU - Art, Jonathan
AU - Alford, Simon
AU - Mann, Christopher
N1 - Funding Information:
Funding: This research was funded by the NIH RO1 NS111749 to S.A. and the NIH R21 DC017292 to J.A., and the National Institutes of Health (123456) and National Science Foundation (456789) to C.M.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9
Y1 - 2021/9
N2 - Stimuli to excitable cells and various cellular processes can cause cell surface defor-mations; for example, when excitable cell membrane potentials are altered during action potentials. However, these cellular changes may be at or below the diffraction limit (in dendrites the structures measured are as small as 1 µm), and imaging by traditional methods is challenging. Using dual lenses incoherent holography lattice light-sheet (IHLLS-2L) detection with holographic phase imaging of selective fluorescent markers, we can extract the full-field cellular morphology or structural changes of the object’s phase in response to external stimulus. This approach will open many new possibilities in imaging neuronal activity and, overall, in light sheet imaging. In this paper, we pre-sent IHLLS-2L as a well-suited technique for quantifying cell membrane deformation in neurons without the actuation of a sample stage or detection microscope objective.
AB - Stimuli to excitable cells and various cellular processes can cause cell surface defor-mations; for example, when excitable cell membrane potentials are altered during action potentials. However, these cellular changes may be at or below the diffraction limit (in dendrites the structures measured are as small as 1 µm), and imaging by traditional methods is challenging. Using dual lenses incoherent holography lattice light-sheet (IHLLS-2L) detection with holographic phase imaging of selective fluorescent markers, we can extract the full-field cellular morphology or structural changes of the object’s phase in response to external stimulus. This approach will open many new possibilities in imaging neuronal activity and, overall, in light sheet imaging. In this paper, we pre-sent IHLLS-2L as a well-suited technique for quantifying cell membrane deformation in neurons without the actuation of a sample stage or detection microscope objective.
KW - Digital holography
KW - Fluorescence microscopy
KW - Lattice light sheet microscopy
KW - Phase modulation
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U2 - 10.3390/photonics8090383
DO - 10.3390/photonics8090383
M3 - Article
AN - SCOPUS:85115031032
SN - 2304-6732
VL - 8
JO - Photonics
JF - Photonics
IS - 9
M1 - 383
ER -