Contribution of multidrug efflux pumps to multiple antibiotic resistance in veterinary clinical isolates of Pseudomonas aeruginosa

Kerry L. Beinlich; Rungtip Chuanchuen; Herbert P. Schweizer

doi:10.1016/S0378-1097(01)00135-5

Contribution of multidrug efflux pumps to multiple antibiotic resistance in veterinary clinical isolates of Pseudomonas aeruginosa

Kerry L. Beinlich, Rungtip Chuanchuen, Herbert P. Schweizer

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The contribution of efflux pumps to multidrug resistance in 12 Pseudomonas aeruginosa isolates from various animal sources was assessed. Western immunoblot analyses demonstrated that all twelve isolates expressed significant levels of the MexAB-OprM efflux system whereas two isolates simultaneously expressed the MexEF-OprN or MexXY systems, respectively. One strain contained a single mutation in mexR, a regulator of mexAB-oprM expression, that did not adversely affect the MexR amino acid sequence, and three isolates contained the same, single base change in the mexA-mexR intergenic region. The MexXY-expressing strain contained two base substitutions in its mexZ regulatory gene which did not alter the MexR sequence.

Original language	English (US)
Pages (from-to)	129-134
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	198
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(01)00135-5
State	Published - May 1 2001
Externally published	Yes

Keywords

Multidrug efflux
Pseudomonas aeruginosa
Veterinary isolate

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(01)00135-5

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title = "Contribution of multidrug efflux pumps to multiple antibiotic resistance in veterinary clinical isolates of Pseudomonas aeruginosa",

abstract = "The contribution of efflux pumps to multidrug resistance in 12 Pseudomonas aeruginosa isolates from various animal sources was assessed. Western immunoblot analyses demonstrated that all twelve isolates expressed significant levels of the MexAB-OprM efflux system whereas two isolates simultaneously expressed the MexEF-OprN or MexXY systems, respectively. One strain contained a single mutation in mexR, a regulator of mexAB-oprM expression, that did not adversely affect the MexR amino acid sequence, and three isolates contained the same, single base change in the mexA-mexR intergenic region. The MexXY-expressing strain contained two base substitutions in its mexZ regulatory gene which did not alter the MexR sequence.",

keywords = "Multidrug efflux, Pseudomonas aeruginosa, Veterinary isolate",

author = "Beinlich, {Kerry L.} and Rungtip Chuanchuen and Schweizer, {Herbert P.}",

note = "Funding Information: This research was supported by a grant from the Research Council of the Colorado State University College of Veterinary Medicine and Biomedical Sciences to H.P.S. R.C. was the recipient of a predoctoral fellowship from the Royal Thai Government. We thank Claudia Gentry-Weeks for providing the clinical isolates and the clinical laboratory antibiotic resistance data. We also acknowledge the gifts of antibodies by N. Gotoh and N. Masuda.",

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doi = "10.1016/S0378-1097(01)00135-5",

language = "English (US)",

volume = "198",

pages = "129--134",

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T1 - Contribution of multidrug efflux pumps to multiple antibiotic resistance in veterinary clinical isolates of Pseudomonas aeruginosa

AU - Beinlich, Kerry L.

AU - Chuanchuen, Rungtip

AU - Schweizer, Herbert P.

N1 - Funding Information: This research was supported by a grant from the Research Council of the Colorado State University College of Veterinary Medicine and Biomedical Sciences to H.P.S. R.C. was the recipient of a predoctoral fellowship from the Royal Thai Government. We thank Claudia Gentry-Weeks for providing the clinical isolates and the clinical laboratory antibiotic resistance data. We also acknowledge the gifts of antibodies by N. Gotoh and N. Masuda.

PY - 2001/5/1

Y1 - 2001/5/1

N2 - The contribution of efflux pumps to multidrug resistance in 12 Pseudomonas aeruginosa isolates from various animal sources was assessed. Western immunoblot analyses demonstrated that all twelve isolates expressed significant levels of the MexAB-OprM efflux system whereas two isolates simultaneously expressed the MexEF-OprN or MexXY systems, respectively. One strain contained a single mutation in mexR, a regulator of mexAB-oprM expression, that did not adversely affect the MexR amino acid sequence, and three isolates contained the same, single base change in the mexA-mexR intergenic region. The MexXY-expressing strain contained two base substitutions in its mexZ regulatory gene which did not alter the MexR sequence.

AB - The contribution of efflux pumps to multidrug resistance in 12 Pseudomonas aeruginosa isolates from various animal sources was assessed. Western immunoblot analyses demonstrated that all twelve isolates expressed significant levels of the MexAB-OprM efflux system whereas two isolates simultaneously expressed the MexEF-OprN or MexXY systems, respectively. One strain contained a single mutation in mexR, a regulator of mexAB-oprM expression, that did not adversely affect the MexR amino acid sequence, and three isolates contained the same, single base change in the mexA-mexR intergenic region. The MexXY-expressing strain contained two base substitutions in its mexZ regulatory gene which did not alter the MexR sequence.

KW - Multidrug efflux

KW - Pseudomonas aeruginosa

KW - Veterinary isolate

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U2 - 10.1016/S0378-1097(01)00135-5

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AN - SCOPUS:0035339126

SN - 0378-1097

VL - 198

SP - 129

EP - 134

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Contribution of multidrug efflux pumps to multiple antibiotic resistance in veterinary clinical isolates of Pseudomonas aeruginosa

Abstract

Keywords

ASJC Scopus subject areas

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