Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

F. Heath Damron; Elizabeth S. McKenney; Mariette Barbier; George W. Liechti; Herbert P. Schweizer; Joanna B. Goldberg

doi:10.1128/AEM.00640-13

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

F. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, George W. Liechti, Herbert P. Schweizer, Joanna B. Goldberg

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Original language	English (US)
Pages (from-to)	4149-4153
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00640-13
State	Published - Jul 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Access to Document

10.1128/AEM.00640-13

Cite this

@article{3493296f9d614b7183011cc6cf06e5ef,

title = "Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis",

abstract = "Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.",

author = "Damron, \{F. Heath\} and McKenney, \{Elizabeth S.\} and Mariette Barbier and Liechti, \{George W.\} and Schweizer, \{Herbert P.\} and Goldberg, \{Joanna B.\}",

year = "2013",

month = jul,

doi = "10.1128/AEM.00640-13",

language = "English (US)",

volume = "79",

pages = "4149--4153",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "13",

}

TY - JOUR

T1 - Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

AU - Damron, F. Heath

AU - McKenney, Elizabeth S.

AU - Barbier, Mariette

AU - Liechti, George W.

AU - Schweizer, Herbert P.

AU - Goldberg, Joanna B.

PY - 2013/7

Y1 - 2013/7

N2 - Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

AB - Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

UR - https://www.scopus.com/pages/publications/84879833328

UR - https://www.scopus.com/inward/citedby.url?scp=84879833328&partnerID=8YFLogxK

U2 - 10.1128/AEM.00640-13

DO - 10.1128/AEM.00640-13

M3 - Article

C2 - 23584769

AN - SCOPUS:84879833328

SN - 0099-2240

VL - 79

SP - 4149

EP - 4153

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 13

ER -

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this