Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

F. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, George W. Liechti, Herbert P. Schweizer, Joanna B. Goldberg

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Original languageEnglish (US)
Pages (from-to)4149-4153
Number of pages5
JournalApplied and Environmental Microbiology
Volume79
Issue number13
DOIs
StatePublished - Jul 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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