Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
|Original language||English (US)|
|Number of pages||5|
|Journal||Applied and Environmental Microbiology|
|State||Published - Jul 2013|
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology