Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

F. Heath Damron; Elizabeth S. McKenney; Mariette Barbier; George W. Liechti; Herbert P. Schweizer; Joanna B. Goldberg

doi:10.1128/AEM.00640-13

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

F. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, George W. Liechti, Herbert P. Schweizer, Joanna B. Goldberg

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Original language	English (US)
Pages (from-to)	4149-4153
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00640-13
State	Published - Jul 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.",

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AU - Damron, F. Heath

AU - McKenney, Elizabeth S.

AU - Barbier, Mariette

AU - Liechti, George W.

AU - Schweizer, Herbert P.

AU - Goldberg, Joanna B.

PY - 2013/7

Y1 - 2013/7

N2 - Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

AB - Wedescribe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

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Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis

Abstract

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