Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

S. E.H. West; H. P. Schweizer; C. Dall; A. K. Sample; L. J. Runyen-Janecky

doi:10.1016/0378-1119(94)90237-2

Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

S. E.H. West
, H. P. Schweizer
, C. Dall
, A. K. Sample
, L. J. Runyen-Janecky

Research output: Contribution to journal › Article › peer-review

520 Scopus citations

Abstract

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

Original language	English (US)
Pages (from-to)	81-86
Number of pages	6
Journal	Gene
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90237-2
State	Published - Oct 11 1994
Externally published	Yes

Keywords

Recombinant DNA
broad-host-range expression vectors
plasmid replication
replication protein

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90237-2

Cite this

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title = "Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa",

abstract = "The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.",

keywords = "Recombinant DNA, broad-host-range expression vectors, plasmid replication, replication protein",

author = "West, \{S. E.H.\} and Schweizer, \{H. P.\} and C. Dall and Sample, \{A. K.\} and Runyen-Janecky, \{L. J.\}",

note = "Funding Information: Grant A131477701AI to S.E.H.W. and by operating grants from the Canadian Cystic Fibrosis Foundation and the Medical Research Council of Canada to H.P.S. H.P.S. is a Medical Scholar of the Medical Research Council of Canada. L.J.R.-J. is a trainee of Public Health Service Grant T32 GM07215. Funding Information: We wish to thank J.P. Leung and N. Goldberg for expert assistance during the initial stages of this project. This research was supported by a grant from the Cystic Fibrosis Foundation and by a Public Health Service",

year = "1994",

month = oct,

day = "11",

doi = "10.1016/0378-1119(94)90237-2",

language = "English (US)",

volume = "148",

pages = "81--86",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

AU - West, S. E.H.

AU - Schweizer, H. P.

AU - Dall, C.

AU - Sample, A. K.

AU - Runyen-Janecky, L. J.

N1 - Funding Information: Grant A131477701AI to S.E.H.W. and by operating grants from the Canadian Cystic Fibrosis Foundation and the Medical Research Council of Canada to H.P.S. H.P.S. is a Medical Scholar of the Medical Research Council of Canada. L.J.R.-J. is a trainee of Public Health Service Grant T32 GM07215. Funding Information: We wish to thank J.P. Leung and N. Goldberg for expert assistance during the initial stages of this project. This research was supported by a grant from the Cystic Fibrosis Foundation and by a Public Health Service

PY - 1994/10/11

Y1 - 1994/10/11

N2 - The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

AB - The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

KW - Recombinant DNA

KW - broad-host-range expression vectors

KW - plasmid replication

KW - replication protein

UR - https://www.scopus.com/pages/publications/0028172499

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DO - 10.1016/0378-1119(94)90237-2

M3 - Article

C2 - 7926843

AN - SCOPUS:0028172499

SN - 0378-1119

VL - 148

SP - 81

EP - 86

JO - Gene

JF - Gene

IS - 1

ER -

Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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