@article{99f835c64e9d4e538f7e62212586957a,
title = "Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa",
abstract = "The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.",
keywords = "Recombinant DNA, broad-host-range expression vectors, plasmid replication, replication protein",
author = "West, {S. E.H.} and Schweizer, {H. P.} and C. Dall and Sample, {A. K.} and Runyen-Janecky, {L. J.}",
note = "Funding Information: Grant A131477701AI to S.E.H.W. and by operating grants from the Canadian Cystic Fibrosis Foundation and the Medical Research Council of Canada to H.P.S. H.P.S. is a Medical Scholar of the Medical Research Council of Canada. L.J.R.-J. is a trainee of Public Health Service Grant T32 GM07215. Funding Information: We wish to thank J.P. Leung and N. Goldberg for expert assistance during the initial stages of this project. This research was supported by a grant from the Cystic Fibrosis Foundation and by a Public Health Service",
year = "1994",
month = oct,
day = "11",
doi = "10.1016/0378-1119(94)90237-2",
language = "English (US)",
volume = "148",
pages = "81--86",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",
}