Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

S. E.H. West, H. P. Schweizer, C. Dall, A. K. Sample, L. J. Runyen-Janecky

Research output: Contribution to journalArticlepeer-review

494 Scopus citations

Abstract

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalGene
Volume148
Issue number1
DOIs
StatePublished - Oct 11 1994
Externally publishedYes

Keywords

  • broad-host-range expression vectors
  • plasmid replication
  • Recombinant DNA
  • replication protein

ASJC Scopus subject areas

  • Genetics

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