The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.
- broad-host-range expression vectors
- plasmid replication
- Recombinant DNA
- replication protein
ASJC Scopus subject areas