Construction of a broad-host-range Tn7-based vector for single-copy PBAD-controlled gene expression in Gram-negative bacteria

F. Heath Damron; Elizabeth S. McKenney; Herbert P. Schweizer; Joanna B. Goldberg

doi:10.1128/AEM.02926-12

Construction of a broad-host-range Tn7-based vector for single-copy P_BAD-controlled gene expression in Gram-negative bacteria

F. Heath Damron
, Elizabeth S. McKenney
, Herbert P. Schweizer
, Joanna B. Goldberg

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacte ia for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

Original language	English (US)
Pages (from-to)	718-721
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	2
DOIs	https://doi.org/10.1128/AEM.02926-12
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacte ia for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.",

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AB - We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacte ia for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

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Construction of a broad-host-range Tn7-based vector for single-copy P_BAD-controlled gene expression in Gram-negative bacteria

Abstract

ASJC Scopus subject areas

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