TY - JOUR
T1 - Construction of a broad-host-range Tn7-based vector for single-copy PBAD-controlled gene expression in Gram-negative bacteria
AU - Damron, F. Heath
AU - McKenney, Elizabeth S.
AU - Schweizer, Herbert P.
AU - Goldberg, Joanna B.
PY - 2013/1
Y1 - 2013/1
N2 - We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacte ia for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.
AB - We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacte ia for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.
UR - http://www.scopus.com/inward/record.url?scp=84871898456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871898456&partnerID=8YFLogxK
U2 - 10.1128/AEM.02926-12
DO - 10.1128/AEM.02926-12
M3 - Article
C2 - 23124231
AN - SCOPUS:84871898456
SN - 0099-2240
VL - 79
SP - 718
EP - 721
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 2
ER -