Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis

Kevin Dybvig, C. Todd French, Le Roy L. Voelker

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Previous attempts to introduce transposon Tn4001 into Mycoplasma pulmonis and Mycoplasma arthritidis have not been successful, possibly due to functional failure of the transposon's gentamicin resistance determinant. Tn4001C and Tn4OO1T were constructed, respectively, by insertion of a chloramphenicol acetyltransferase gene and the tetM tetracycline resistance determinant into Tn4001. Both Tn4001C and Tn4OO1T transposed in M. pulmonis, and Tn4OO1T transposed in M. arthritidis. The incorporation of a Tn4OO1T derivative that contained lacZ into either Mycoplasma species resulted in transformants with readily detectable LacZ activity. Tn4OO1T may be of general utility for use as a mycoplasma cloning vehicle because tetM functions in all species of Mycoplasma examined thus far.

Original languageEnglish (US)
Pages (from-to)4343-4347
Number of pages5
JournalJournal of Bacteriology
Volume182
Issue number15
DOIs
StatePublished - Aug 2000
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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