TY - JOUR
T1 - Computationally optimizing the compliance of multilayered biomimetic tissue engineered vascular grafts
AU - Tamimi, Ehab A.
AU - Ardila, Diana Catalina
AU - Ensley, Burt D.
AU - Kellar, Robert S.
AU - Vande Geest, Jonathan P.
N1 - Funding Information:
• National Institutes of Health (Grant No. 1R56HL136517-01; Funder ID: 10.13039/100000002).
Funding Information:
This research was funded by the NIH, grant 1R56HL136517-01 to JPVG. Additional support for E.A. Tamimi was also provided by the National Institute of Biomedical Imaging and Bioengineering under award number EB003392. We would also like to acknowledge Scott Harrison for his important contributions to the optimization scheme used in this work.
Publisher Copyright:
© 2019 American Society of Mechanical Engineers (ASME). All rights reserved.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Coronary artery bypass grafts used to treat coronary artery disease (CAD) often fail due to compliance mismatch. In this study, we have developed an experimental/computational approach to fabricate an acellular biomimetic hybrid tissue engineered vascular graft (TEVG) composed of alternating layers of electrospun porcine gelatin/polycaprolactone (PCL) and human tropoelastin/PCL blends with the goal of compliance-matching to rat abdominal aorta, while maintaining specific geometrical constraints. Polymeric blends at three different gelatin:PCL (G:PCL) and tropoelastin:PCL (T:PCL) ratios (80:20, 50:50, and 20:80) were mechanically characterized. The stress-strain data were used to develop predictive models, which were used as part of an optimization scheme that was implemented to determine the ratios of G:PCL and T:PCL and the thickness of the individual layers within a TEVG that would compliance match a target compliance value. The hypocompliant, isocompliant, and hypercompliant grafts had target compliance values of 0.000256, 0.000568, and 0.000880mmHg-1, respectively. Experimental validation of the optimization demonstrated that the hypercompliant and isocompliant grafts were not statistically significant from their respective target compliance values (p-value=0.37 and 0.89, respectively). The experimental compliance values of the hypocompliant graft were statistically significant than their target compliance value (p-value=0.047). We have successfully demonstrated a design optimization scheme that can be used to fabricate multilayered and biomimetic vascular grafts with targeted geometry and compliance.
AB - Coronary artery bypass grafts used to treat coronary artery disease (CAD) often fail due to compliance mismatch. In this study, we have developed an experimental/computational approach to fabricate an acellular biomimetic hybrid tissue engineered vascular graft (TEVG) composed of alternating layers of electrospun porcine gelatin/polycaprolactone (PCL) and human tropoelastin/PCL blends with the goal of compliance-matching to rat abdominal aorta, while maintaining specific geometrical constraints. Polymeric blends at three different gelatin:PCL (G:PCL) and tropoelastin:PCL (T:PCL) ratios (80:20, 50:50, and 20:80) were mechanically characterized. The stress-strain data were used to develop predictive models, which were used as part of an optimization scheme that was implemented to determine the ratios of G:PCL and T:PCL and the thickness of the individual layers within a TEVG that would compliance match a target compliance value. The hypocompliant, isocompliant, and hypercompliant grafts had target compliance values of 0.000256, 0.000568, and 0.000880mmHg-1, respectively. Experimental validation of the optimization demonstrated that the hypercompliant and isocompliant grafts were not statistically significant from their respective target compliance values (p-value=0.37 and 0.89, respectively). The experimental compliance values of the hypocompliant graft were statistically significant than their target compliance value (p-value=0.047). We have successfully demonstrated a design optimization scheme that can be used to fabricate multilayered and biomimetic vascular grafts with targeted geometry and compliance.
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U2 - 10.1115/1.4042902
DO - 10.1115/1.4042902
M3 - Article
C2 - 30778568
AN - SCOPUS:85064883178
SN - 0148-0731
VL - 141
JO - Journal of Biomechanical Engineering
JF - Journal of Biomechanical Engineering
IS - 6
M1 - 061003
ER -