Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Mirjam Kaestli; Leisha J. Richardson; Rebecca E. Colman; Apichai Tuanyok; Erin P. Price; Jolene R. Bowers; Mark Mayo; Erin Kelley; Meagan L. Seymour; Derek S. Sarovich; Talima Pearson; David M. Engelthaler; David M. Wagner; Paul S. Keim; James M. Schupp; Bart J. Currie

doi:10.1128/JCM.06737-11

Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Mirjam Kaestli, Leisha J. Richardson, Rebecca E. Colman, Apichai Tuanyok, Erin P. Price, Jolene R. Bowers, Mark Mayo, Erin Kelley, Meagan L. Seymour, Derek S. Sarovich, Talima Pearson, David M. Engelthaler, David M. Wagner, Paul S. Keim, James M. Schupp, Bart J. Currie

Biological Sciences

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44 Scopus citations

Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

Original language	English (US)
Pages (from-to)	2059-2062
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1128/JCM.06737-11
State	Published - Jun 2012

ASJC Scopus subject areas

Microbiology (medical)

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Kaestli, M., Richardson, L. J., Colman, R. E., Tuanyok, A., Price, E. P., Bowers, J. R., Mayo, M., Kelley, E., Seymour, M. L., Sarovich, D. S., Pearson, T., Engelthaler, D. M., Wagner, D. M., Keim, P. S., Schupp, J. M., & Currie, B. J. (2012). Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. Journal of Clinical Microbiology, 50(6), 2059-2062. https://doi.org/10.1128/JCM.06737-11

Kaestli, M, Richardson, LJ, Colman, RE, Tuanyok, A, Price, EP, Bowers, JR, Mayo, M, Kelley, E, Seymour, ML, Sarovich, DS, Pearson, T, Engelthaler, DM, Wagner, DM, Keim, PS, Schupp, JM & Currie, BJ 2012, 'Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens', Journal of Clinical Microbiology, vol. 50, no. 6, pp. 2059-2062. https://doi.org/10.1128/JCM.06737-11

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title = "Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens",

abstract = "Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.",

author = "Mirjam Kaestli and Richardson, {Leisha J.} and Colman, {Rebecca E.} and Apichai Tuanyok and Price, {Erin P.} and Bowers, {Jolene R.} and Mark Mayo and Erin Kelley and Seymour, {Meagan L.} and Sarovich, {Derek S.} and Talima Pearson and Engelthaler, {David M.} and Wagner, {David M.} and Keim, {Paul S.} and Schupp, {James M.} and Currie, {Bart J.}",

year = "2012",

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doi = "10.1128/JCM.06737-11",

language = "English (US)",

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AU - Kaestli, Mirjam

AU - Richardson, Leisha J.

AU - Colman, Rebecca E.

AU - Tuanyok, Apichai

AU - Price, Erin P.

AU - Bowers, Jolene R.

AU - Mayo, Mark

AU - Kelley, Erin

AU - Seymour, Meagan L.

AU - Sarovich, Derek S.

AU - Pearson, Talima

AU - Engelthaler, David M.

AU - Wagner, David M.

AU - Keim, Paul S.

AU - Schupp, James M.

AU - Currie, Bart J.

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AB - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

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Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

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