@article{e6e2cd8ad7d744f587b48960a359911d,
title = "Choline catabolism, σ54 factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene",
abstract = "Choline favors the pathogenesis of Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of choline and the gene coding for PchP. We have also identified and characterized the promoter of the pchP gene, its transcriptional organization and the factors that affect its expression. Deletion analyses reveal that the region between -188 and -68 contains all controlling elements necessary for pchP expression: a hypothetical -12/-24 promoter element, a consensus sequence for the integration host factor (-141/-133), and a palindromic sequence resembling a binding site for a potential enhancer binding protein (-190/-174). Our data also demonstrate that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of pchP and is partially dependent on σ54 factor.",
keywords = "Choline, Phosphorylcholine phosphatase, Pseudomonas aeruginosa, Regulation",
author = "Massimelli, {Maria J.} and S{\'a}nchez, {Diego G.} and Buchieri, {Maria V.} and Leticia Olvera and Beassoni, {Paola R.} and Schweizer, {Herbert P.} and Enrique Morett and Lisa, {Angela T.}",
note = "Funding Information: We are very grateful to Dr C.E. Domenech for fruitful discussion during the course of this study, especially in the preparation of the manuscript. We also thank Dr. P. Gayt{\'a}n and J. Ya{\~n}ez for technical support, Dr. D. Haas for providing the PAO6358 and PAO6360 strains, and R. Grande for helpful advice. Financial support: National Council of Scientific and Technological Research (CONICET) ; National Agency of Scientific and Technological Promotion (ANPCYT; grant PICT 25523/04 BID 1728 OC-AR) and the Scientific and Technological Secretary of The National University of Rio Cuarto (SECYT/UNRC), Argentina . Work in EM's laboratory was supported by NIH grant GM071962-03 and CONACYT grant 83686 G.I. Part of this work was performed in the laboratory of HPS and was supported by an international Fellowship from the American Society for Microbiology to MJM. MJM had a fellowship from CONICET but her present address is: HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA. PRB has a fellowship from CONICET, and ATL is a member of the Research Career of CONICET.",
year = "2011",
month = jul,
day = "20",
doi = "10.1016/j.micres.2010.07.004",
language = "English (US)",
volume = "166",
pages = "380--390",
journal = "Microbiological Research",
issn = "0944-5013",
publisher = "Urban und Fischer Verlag Jena",
number = "5",
}