Characterization of Molecular Mechanisms Controlling fabAB Transcription in Pseudomonas aeruginosa

Herbert P. Schweizer, Kyoung Hee Choi

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background: The FabAB pathway is one of the unsaturated fatty acid (UFA) synthesis pathways for Pseudomonas aeruginosa. It was previously noted that this operon was upregulated in biofilms and repressed by exogenous UFAs. Deletion of a 30 nt fabA upstream sequence, which is conserved in P. aeruginosa, P. putida, and P. syringae, led to a significant decrease in fabA transcription, suggesting positive regulation by an unknown positive regulatory mechanism. Methods/Principal Findings: Here, genetic and biochemical approaches were employed to identify a potential fabAB activator. Deletion of candidate genes such as PA1611 or PA1627 was performed to determine if any of these gene products act as a fabAB activator. However, none of these genes were involved in the regulation of fabAB transcription. Use of mariner-based random mutagenesis to screen for fabA activator(s) showed that several genes encoding unknown functions, rpoN and DesA may be involved in fabA regulation, but probably via indirect mechanisms. Biochemical attempts performed did fail to isolate an activator of fabAB operon. Conclusion/Significance: The data suggest that fabA expression might not be regulated by protein-binding, but by a distinct mechanism such as a regulatory RNA-based mechanism.

Original languageEnglish (US)
Article numbere45646
JournalPLoS ONE
Issue number10
StatePublished - Oct 2 2012
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General


Dive into the research topics of 'Characterization of Molecular Mechanisms Controlling fabAB Transcription in Pseudomonas aeruginosa'. Together they form a unique fingerprint.

Cite this