Challenges and Hallmarks of Establishing Alkylacetylphosphonates as Probes of Bacterial 1-Deoxy- d -xylulose 5-Phosphate Synthase

Keyphrases

• 1-deoxy-D-xylulose-5-phosphate Synthase 100%
• 1-Deoxy-d-xylulose 5-phosphate 25%
• Thiamine Diphosphate 25%
• Antimicrobial Activity 12%
• Isoprenoids 12%
• Methylerythritol Phosphate Pathway 12%
• Microenvironment 12%
• Active Sites 12%
• Branch Point 12%
• Efflux 12%
• Metabolic 12%
• Bacterial Growth 12%
• Approaches to Study 12%
• Glyceraldehyde-3-phosphate 12%
• Pyridoxal 5′-phosphate 12%
• Nutrient-rich Conditions 12%
• Growing Media 12%
• Target Engagement 12%
• Growth Environment 12%
• Inhibitory Activity 12%
• Screening Conditions 12%
• Central Metabolism 12%
• Sterically Demanding 12%
• Minimum Inhibitory Concentration 12%
• Antimicrobial Screening 12%
• Thiamine Supplementation 12%
• Inhibitor Potency 12%
• AcrAB-TolC 12%
• Intracellular Targets 12%
• Alkyl Chain Length 12%
• Supplementation Studies 12%
• Suboptimal Growth 12%
• Sequential Mechanisms 12%

Biochemistry, Genetics and Molecular Biology

• Synthase 100%
• Xylulose 5-Phosphate 100%
• Thiamine Pyrophosphate 20%
• Metabolic Pathway 10%
• Antimicrobial Activity 10%
• Anabolism 10%
• Active Site 10%
• Precursor 10%
• Glyceraldehyde 3-Phosphate 10%
• Bacterial Growth 10%
• Minimum Inhibitory Concentration 10%
• Thiamine 10%
• Vitamin B1 10%
• Pyridoxal Phosphate 10%