Carbon management practices regulate soil bacterial communities in response to nitrogen addition in a pine forest

Lu Yang, Na Wang, Ye Chen, Wen Yang, Dashuan Tian, Chunyu Zhang, Xiuhai Zhao, Jinsong Wang, Shuli Niu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Carbon (C) and nitrogen (N) represent the commonest limiting nutrients for microbial growth in terrestrial ecosystems. However, most of our understanding of how C:N ratios modulate microbial growth comes from short-term growth assays under controlled conditions. Methods: Four levels of N additions including control (CK, 0 kg·hm− 2·a− 1), low N (LN, 50 kg·hm− 2·a− 1), medium N (MN, 100 kg·hm− 2·a− 1), and high N (HN, 150 kg·hm− 2·a− 1) inputs were applied monthly in a pine forest (Pinus tabulaeformis). Three C management practices were further conducted in each N additions plot, namely aboveground litter and belowground root removal (LRR), aboveground litter removal (LR), and intact soils (non-removal, NR). Soil bacterial richness, diversity, community composition and soil properties were measured. Results: MN and HN significantly increased the relative abundance of copiotrophic taxa, but decreased that of oligotrophic taxa. Bacterial richness and diversity were not altered by N enrichment. LRR significantly increased the relative abundance of Gemmatimonadetes while decreased that of Actinobacteria. C management did not affect bacterial richness and community structure but LR significantly decreased bacterial diversity. Structural equation modeling showed that N addition induced the decrease in soil pH was responsible for the changes in the bacterial community structure. C management exhibited a direct negative effect on bacterial diversity and had an indirect positive effect on it via increasing soil moisture and microbial biomass C:N ratio. Conclusions: The findings highlight the contrasting impacts of N addition and C management on soil bacterial communities and emphasize the regulation of above- and below-ground C substrate supplies on the N responses of soil bacterial communities.

Original languageEnglish (US)
Pages (from-to)137-151
Number of pages15
JournalPlant and Soil
Volume452
Issue number1-2
DOIs
StatePublished - Jul 1 2020

Keywords

  • Aboveground litter removal
  • Bacterial diversity
  • Bacterial richness
  • Community structure
  • Nitrogen addition
  • Root removal
  • Soil microbial biomass C:N ratio

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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