TY - JOUR
T1 - Carbohydrate-binding plasma proteins from the gastropod Biomphalaria glabrata
T2 - Strain specificity and the effects of trematode infection
AU - Monroy, Fernando
AU - Hertel, Lynn A.
AU - Loker, Eric S.
N1 - Funding Information:
Acknowledgements--We thank Ms. Lee Couch for expert technical assistance, and Dr. Christopher J. Bayne and Dr. Sarah E. Fryer for critically reading the manuscript. This study was supported by NIH grant AI-24340.
PY - 1992
Y1 - 1992
N2 - As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.
AB - As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.
KW - Biomphalaria glabrata
KW - Comparative immunobiology
KW - Digenetic trematodes
KW - Echinostoma paraensei
KW - Hemolymph proteins
KW - Lectins
KW - Molluscs
KW - Schistosoma mansoni
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U2 - 10.1016/0145-305X(92)90038-E
DO - 10.1016/0145-305X(92)90038-E
M3 - Article
C2 - 1330758
AN - SCOPUS:0026675339
SN - 0145-305X
VL - 16
SP - 355
EP - 366
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 5
ER -