TY - JOUR
T1 - Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex
AU - Tuanyok, Apichai
AU - Mayo, Mark
AU - Scholz, Holger
AU - Hall, Carina M.
AU - Allender, Christopher J.
AU - Kaestli, Mirjam
AU - Ginther, Jennifer
AU - Spring-Pearson, Senanu
AU - Bollig, Molly C.
AU - Stone, Joshua K.
AU - Settles, Erik W.
AU - Busch, Joseph D.
AU - Sidak-Loftis, Lindsay
AU - Sahl, Jason W.
AU - Thomas, Astrid
AU - Kreutzer, Lisa
AU - Georgi, Enrico
AU - Gee, Jay E.
AU - Bowen, Richard A.
AU - Ladner, Jason T.
AU - Lovett, Sean
AU - Koroleva, Galina
AU - Palacios, Gustavo
AU - Wagner, David M.
AU - Currie, Bart J.
AU - Keim, Paul
N1 - Publisher Copyright:
© 2017 Tuanyok et al.
PY - 2017
Y1 - 2017
N2 - During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).
AB - During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).
KW - Burkholderia humptydooensis sp. nov.
KW - Burkholderia pseudomallei complex
KW - MSMB43
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UR - http://www.scopus.com/inward/citedby.url?scp=85013382520&partnerID=8YFLogxK
U2 - 10.1128/AEM.02802-16
DO - 10.1128/AEM.02802-16
M3 - Article
C2 - 27986727
AN - SCOPUS:85013382520
SN - 0099-2240
VL - 83
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 5
M1 - e02802-16
ER -