TY - JOUR
T1 - Binding of bivalent ligand to cell surface IgE
T2 - Can one detect ring formation?
AU - Posner, Richard G.
AU - Dembo, Micah
PY - 1994/12
Y1 - 1994/12
N2 - It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond to the size (Fewtrell and Metzger (1980) J. Immun. 125, 701-710) as well as the stereochemistry (Ortega et al. (1989) Eur. J. Immun. 19, 2251-2256) of receptor aggregates. One approach to arriving at data relevant to this question has been to construct simple bivalent ligands that can bind to surface immunoglobulin. Several authors have suggested that when these bivalent ligands interact with surface immunoglobulin the formation of small stable cyclic complexes is highly favored. In this paper we consider whether it is possible to completely determine the parameters that describe the binding of a bivalent ligand to a bivalent receptor with the available experimental technology. We show that with the appropriate analysis procedure, using a modified equivalent site model, these parameters can be reliably determined from only three experiments even when there is a large amount of ring formation.
AB - It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond to the size (Fewtrell and Metzger (1980) J. Immun. 125, 701-710) as well as the stereochemistry (Ortega et al. (1989) Eur. J. Immun. 19, 2251-2256) of receptor aggregates. One approach to arriving at data relevant to this question has been to construct simple bivalent ligands that can bind to surface immunoglobulin. Several authors have suggested that when these bivalent ligands interact with surface immunoglobulin the formation of small stable cyclic complexes is highly favored. In this paper we consider whether it is possible to completely determine the parameters that describe the binding of a bivalent ligand to a bivalent receptor with the available experimental technology. We show that with the appropriate analysis procedure, using a modified equivalent site model, these parameters can be reliably determined from only three experiments even when there is a large amount of ring formation.
KW - Monte Carlo analysis
KW - ring formation
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U2 - 10.1016/0161-5890(94)90160-0
DO - 10.1016/0161-5890(94)90160-0
M3 - Article
C2 - 7823970
AN - SCOPUS:0028096718
SN - 0161-5890
VL - 31
SP - 1439
EP - 1445
JO - Molecular Immunology
JF - Molecular Immunology
IS - 18
ER -