TY - JOUR
T1 - Baleen whale microsatellite panel for individual identification and parentage assignment in Mysticeti
AU - Suárez-Menéndez, Marcos
AU - Bérubé, Martine
AU - Bachmann, Lutz
AU - Best, Peter
AU - Davison, Nick
AU - Heide-Jørgensen, Mads Peter
AU - Lesage, Véronique
AU - Oosting, Tom
AU - Prieto, Rui
AU - Ramp, Christian
AU - Robbins, Jooke
AU - Sears, Richard
AU - Silva, Mónica A.
AU - ten Doeschate, Mariel T.I.
AU - Tollis, Marc
AU - Vermeulen, Els
AU - Víkingsson, Gísli A.
AU - Wiig, Øystein
AU - Palsbøll, Per J.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/6
Y1 - 2024/6
N2 - Highly polymorphic single tandem repeat loci (STR, also known as microsatellite loci) remain a familiar, cost efficient class of genetic markers in genetic studies in ecology, behavior and conservation. Here we characterize a new, universal set of ten STR loci in seven species of baleen whales, optimized for PCR amplification in two multiplex reactions along with a Y chromosome marker for sex determination. The optimized, universal set of STR loci provides a convenient starting point for new genetic studies in baleen whales aimed at identifying individuals and populations. Data from the new STR loci were combined with genotypes from previously published STR loci to assess the power to assign parentage using paternity exclusion in four species: fin whale (Balaenoptera physalus), humpback whale (Megaptera novaeangliae), blue whale (B. musculus) and bowhead whale (Balaena mysticetus). Our results suggest that parentage studies should always be accompanied by a power analysis in order to ascertain that each individual specific study is based upon data with sufficient power to assign parentage with statistical rigor.
AB - Highly polymorphic single tandem repeat loci (STR, also known as microsatellite loci) remain a familiar, cost efficient class of genetic markers in genetic studies in ecology, behavior and conservation. Here we characterize a new, universal set of ten STR loci in seven species of baleen whales, optimized for PCR amplification in two multiplex reactions along with a Y chromosome marker for sex determination. The optimized, universal set of STR loci provides a convenient starting point for new genetic studies in baleen whales aimed at identifying individuals and populations. Data from the new STR loci were combined with genotypes from previously published STR loci to assess the power to assign parentage using paternity exclusion in four species: fin whale (Balaenoptera physalus), humpback whale (Megaptera novaeangliae), blue whale (B. musculus) and bowhead whale (Balaena mysticetus). Our results suggest that parentage studies should always be accompanied by a power analysis in order to ascertain that each individual specific study is based upon data with sufficient power to assign parentage with statistical rigor.
KW - Microsatellite
KW - Multiplex
KW - Mysticeti
KW - Paternity
KW - STR loci
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U2 - 10.1016/j.gecco.2024.e02947
DO - 10.1016/j.gecco.2024.e02947
M3 - Article
AN - SCOPUS:85190136832
SN - 2351-9894
VL - 51
JO - Global Ecology and Conservation
JF - Global Ecology and Conservation
M1 - e02947
ER -